HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.

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Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns

Use these endcapped, ultrapure, silica-based columns to generate exceptional peak shape and resolution for HPLC and LC/MS analyses using generic gradients

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Thermo Scientific™ Hypersil™ ODS C18 Columns

Use as a general purpose C18 phase for acidic and neutral analytes.

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Pricing and Availability
Specifications

Pore Size	120 Å
Carbon Load	10%
Stationary Phase	C18
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Column Type	Reversed Phase
pH	2 to 8
Surface Area	170 m²/g
USP Type	L1

Thermo Scientific™ Accucore™ C18 HPLC Columns

Use this column, with its specially designed solid core, for a broad range of nonpolar analytes across multiple applications.

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Pricing and Availability
Specifications

Pore Size	80 Å
Particle Size	2.6 μm
Carbon Load	9%
Stationary Phase	C18
Temperature	70°C
Phase	Reversed Phase
Endcapped	Yes
Column Type	Reversed Phase
pH	1 to 11
Surface Area	130 m²/g
Particle Shape	Spherical Solid Core
USP Type	L1

Thermo Scientific™ Acclaim™ 120 C18 Columns

Use these high-efficiency reversed phase C18 columns for maximum resolution in a wide variety of applications.

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Pricing and Availability
Specifications

Pore Size	120 Å
Carbon Load	18%
Stationary Phase	C18
Temperature	60°C
Endcapped	Yes
Column Type	Reversed Phase
pH	2 to 8
Surface Area	300 m²/g
USP Type	L1

Thermo Scientific™ Hypersil™ BDS C18 Columns

Use as a robust, general-purpose column for a wide range of applications in QC/QA labs.

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Pricing and Availability
Specifications

Carbon Load	11%
Stationary Phase	C18
Phase	Reversed Phase
Endcapped	Yes
Column Type	Reversed Phase
Surface Area	170 m²/g
USP Type	L1

Macherey-Nagel™ Nucleosil™ 100-5 Nautilus Analytical HPLC Standard EC Column

Pricing and Availability
Specifications

Pore Size	100 Å
Solvents	Acetonitrile/water
For Use With	HPLC
Endcapped	Yes
Quantity	1 Ea.
Crosslinking	Octadecyl
Column Chemistry	Silica
Column Format	Analytical Column
Particle Size	5 μm
Type	Analytical EC Column
Carbon Load	16%
Packing Material	Silica
Mobile Phase Compatibility	Acetonitrile/water
pH	2 to 8

Thermo Scientific™ Hypersil™ ODS-2 C18 Columns

Use as a general purpose C18 phase for acidic and neutral analytes separated under difficult operating conditions.

Pricing and Availability
Specifications

Pore Size	80 Å
Carbon Load	11%
Stationary Phase	C18
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Packing Material	Spherical, Fully Porous Type A Silica
Column Type	Reversed Phase
pH	2 to 8
Surface Area	220 m²/g
Max. Pressure	5800 psi (400 bar)
Product Line	Hypersil Classical
USP Type	L1

Thermo Scientific™ ProPac™ 3R SCX 3 μm HPLC Columns

Get the performance you need with ProPac™ 3R SCX HPLC columns. Suitable for charge variant separations and recommended for therapeutic proteins, mAbs, ADCs, and biosimilar proteins.

Pricing and Availability
Specifications

Pore Size	Nonporous
Particle Size	3 μm
Stationary Phase	Proprietary
Temperature	Ambient to 60°C
Phase	Strong Cation Exchange
Packing Material	Monodisperse Polymer Resin
Column Type	Ion Exchange
Mobile Phase Compatibility	Recommended for use with CX-1 pH buffers
pH	2 to 12
Technique	Bio-LC
Max. Pressure	4500 psi (310 bar)
Product Line	ProPac
Column Format	Analytical Column

Thermo Scientific™ Accucore™ 150 Amide HILIC HPLC Columns

Designed for the separation of hydrophilic biomolecules in HILIC mode—an excellent choice for glycan separations.

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Thermo Scientific™ BioBasic™ AX HPLC Columns

Use Thermo Scientific™ BioBasic™ AX LC Columns for the separation of proteins, peptides, other anionic species and highly polar molecules.

Pricing and Availability
Specifications

Pore Size	300 Å
Particle Size	5 μm
Carbon Load	3%
Stationary Phase	Polyethyleneimine
Temperature	60°C
pH	2 to 8
Surface Area	100 m²/g
Max. Pressure	5800 psi (400 bar)

Thermo Scientific™ Acclaim™ SEC-300 Size Exclusion Chromatography HPLC Columns

Separate water-soluble polymers and oligomers with the Acclaim family of resin-based, high-performance, size-exclusion chromatography columns.

Pricing and Availability
Specifications

Pore Size	300 Å
Stationary Phase	Proprietary
Temperature	60°C
Phase	Size Exclusion
Endcapped	Yes
Column Type	Size Exclusion
Quantity	Each
Green Features	Responsibly packaged
Particle Size	5 μm
pH	2 to 12
Technique	Bio-LC
Particle Shape	Spherical
Product Line	Acclaim
USP Type	N/A

Thermo Scientific™ SurePac™ Bio 550 SEC MDi™ HPLC Columns

Experience the future of Size-Exclusion Chromatography (SEC) with SurePac™ Bio 550 SEC MDi™ analytical columns and guards exclusively designed to deliver robust and reproducible performance at high resolution and throughput to accelerate your therapeutics analysis process.

Pricing and Availability
Specifications

Particle Size	3 μm
Stationary Phase	Proprietary
Temperature	20°C to 60°C
Phase	Size Exclusion
Packing Material	Monodisperse Silica Resin
pH	2 to 8
Quantity	1 Ea.
Technique	SEC

Thermo Scientific™ BioBasic™ PREP SEC 120 Size Exclusion Chromatography HPLC Columns

Achieve outstanding size separation of water soluble compounds with BioBasic™ PREP SEC 120 Size Exclusion Chromatography HPLC Columns.

Pricing and Availability
Specifications

Pore Size	120 Å
Stationary Phase	Diol (OH)
Temperature	60°C
Phase	Size Exclusion
For Use With	Preparative Size Exclusion
Endcapped	No
Column Type	Size Exclusion
For Use With (Application)	Preparative Chromatography
Surface Area	300 m²/g
Max. Pressure	4300 psi (295 bar)
Particle Size	5 μm
Carbon Load	>8.2%
Packing Material	Spherical, Fully Porous, Ultrapure Silica
Diameter (Metric)	7.8 mm
pH	2 to 8
USP Type	L59

Thermo Scientific™ BioBasic™ SCX HPLC Columns

BioBasic SCX LC Columns are optimized for the separation of proteins, peptides and other cationic species.

Pricing and Availability
Specifications

Pore Size	300 Å
Particle Size	5 μm
Carbon Load	3%
Stationary Phase	Sulfonic Acid
Temperature	60°C
Phase	Ion Exchange
Endcapped	No
Column Type	Ion Exchange
pH	2 to 8
Surface Area	100 m²/g
Max. Pressure	5800 psi (400 bar)
Product Line	BioBasic
USP Type	L52

Thermo Scientific™ Acclaim™ 300 C18 HPLC Columns

Use these wide-pore C18 columns to achieve high-resolution protein separations.

Pricing and Availability
Specifications

Pore Size	300 Å
Particle Size	3 μm
Carbon Load	8%
Stationary Phase	C18
Temperature	60°C
For Use With	LC/MS
Endcapped	Yes
Column Type	Reversed Phase
pH	2.5 to 7.5
Green Features	Responsibly packaged
Surface Area	100 m²/g
Product Line	Acclaim
USP Type	L1

HPLC Columns

HPLC Columns

Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns

Thermo Scientific™ Hypersil™ ODS C18 Columns

Thermo Scientific™ Accucore™ C18 HPLC Columns

Thermo Scientific™ Acclaim™ 120 C18 Columns

Thermo Scientific™ Hypersil™ BDS C18 Columns

Macherey-Nagel™ Nucleosil™ 100-5 Nautilus Analytical HPLC Standard EC Column

Thermo Scientific™ Hypersil™ ODS-2 C18 Columns

Thermo Scientific™ ProPac™ 3R SCX 3 μm HPLC Columns

Thermo Scientific™ Accucore™ 150 Amide HILIC HPLC Columns

Thermo Scientific™ BioBasic™ AX HPLC Columns

Thermo Scientific™ Acclaim™ SEC-300 Size Exclusion Chromatography HPLC Columns

Thermo Scientific™ SurePac™ Bio 550 SEC MDi™ HPLC Columns

Thermo Scientific™ BioBasic™ PREP SEC 120 Size Exclusion Chromatography HPLC Columns

Thermo Scientific™ BioBasic™ SCX HPLC Columns

Thermo Scientific™ Acclaim™ 300 C18 HPLC Columns

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Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns SureTRACESupports traceability with guaranteed access to certificates and proactive change notifications.Learn More

Thermo Scientific™ Accucore™ C18 HPLC Columns SureTRACESupports traceability with guaranteed access to certificates and proactive change notifications.Learn More

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Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns

Thermo Scientific™ Accucore™ C18 HPLC Columns