Reverse Transcription Products
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Reverse Transcription Products
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Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit
The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction.
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Shipping Condition | Dry Ice |
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Format | Kit |
Reverse Transcriptase | MultiScribe |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 120 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Concentration | 50 U/μL |
Technique | Reverse Transcription |
Starting Material | RNA |
Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit
The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.
Shipping Condition | Dry Ice |
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Format | Kit |
Reverse Transcriptase | MultiScribe |
Size (Final Product) | Up to 7 kb |
Reaction Format | Premixed Components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 30 to 60 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Optimal Reaction Temperature | 37°C |
Technique | Reverse Transcription |
Starting Material | RNA |
For Use With (Application) | Real Time PCR (qPCR),RT-PCR |
Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit
The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes.
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Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript III |
Final Product Type | First-Strand cDNA |
For Use With (Application) | Real Time PCR (qPCR) |
Detection Method | qPCR |
Format | Kit |
Size (Final Product) | Up to 10 kb |
Reaction Format | Master Mix |
Reaction Speed | 60 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogen™ SuperScript™ IV VILO™ Master Mix
SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript IV |
Final Product Type | First-Strand cDNA |
Concentration | 5X |
For Use With (Application) | Real Time PCR (qPCR) |
Detection Method | qPCR |
Format | Master Mix |
Size (Final Product) | Up to 10 kb |
Reaction Format | Master Mix |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Thermo Scientific™ Maxima First Strand cDNA Synthesis Kit for RT-qPCR
The Thermo Scientific Maxima First Strand cDNA Synthesis Kit for RT-qPCR, available with or without dsDNase, is a convenient system optimized for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | Maxima |
Final Product Type | First-Strand cDNA |
For Use With (Application) | Real Time PCR (qPCR) |
Format | Kit |
Size (Final Product) | Up to 20 kb |
Reaction Format | Separate components |
Reaction Speed | 30 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Optimal Reaction Temperature | 50°C to 55°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogen™ SuperScript™ IV First-Strand Synthesis System
The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
GC-Rich PCR Performance | High |
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Format | Kit |
Reverse Transcriptase | SuperScript IV |
Size (Final Product) | Up to 12.3 kb |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogen™ SuperScript™ III CellsDirect™ cDNA Synthesis Kit
The SuperScript III CellsDirect cDNA Synthesis Kit is optimized for synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating the RNA.
GC-Rich PCR Performance | High |
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Format | Kit |
Reverse Transcriptase | SuperScript III |
Size (Final Product) | Up to 4.5 kb |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 50 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | Cells |
For Use With (Application) | Real Time PCR (qPCR) |
Invitrogen™ SuperScript™ IV Reverse Transcriptase
SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions.
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Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript IV |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/μL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 12.3 kb |
Reaction Format | Separate components |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogen™ SuperScript™ III Reverse Transcriptase
Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript III |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/μL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 12.3 kb |
Reaction Format | Separate components |
Reaction Speed | 30 to 50 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogen™ SuperScript™ II Reverse Transcriptase
Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Shipping Condition | Wet Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript II |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/μL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 12.3 kb |
Reaction Format | Separate components |
Reaction Speed | 50 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit
Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.
Shipping Condition | Dry Ice |
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Content And Storage | • RevertAid Reverse Transcriptase |
Reverse Transcriptase | RevertAid |
Final Product Type | First-Strand cDNA |
For Use With (Application) | RT-PCR |
Format | Kit |
Size (Final Product) | Up to 13 kb |
Reaction Format | Separate components |
Reaction Speed | 60 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Thermo Scientific™ RevertAid H Minus First Strand cDNA Synthesis Kit
Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.
Shipping Condition | Dry Ice |
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Format | Kit |
Reverse Transcriptase | RevertAid H Minus |
Size (Final Product) | Up to 13 kb |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 60 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 42°C to 45°C |
Technique | Reverse Transcription |
Starting Material | RNA |
For Use With (Application) | Real Time PCR (qPCR),RT-PCR |
Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)
Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties.
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Shipping Condition | Dry Ice |
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Content And Storage | • RevertAid Reverse Transcriptase (200 U/μL) |
Reverse Transcriptase | RevertAid |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/μL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 13 kb |
Reaction Format | Separate components |
Reaction Speed | 60 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Thermo Scientific™ Maxima H Minus Reverse Transcriptase (200 U/μL)
Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | Maxima H Minus |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/μL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 20 kb |
Reaction Format | Separate components |
Reaction Speed | 15 to 30 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C to 55°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogen™ M-MLV Reverse Transcriptase (200 U/μL)
M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid.
Shipping Condition | Wet Ice |
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Format | Stand-alone Enzyme |
Reverse Transcriptase | M-MLV |
Size (Final Product) | Up to 7 kb |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 50 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Concentration | 200 U/μL |
Optimal Reaction Temperature | 37°C |
Technique | Reverse Transcription |
Starting Material | RNA |