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Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats
Buffer | Sample Loading Buffers |
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Content And Storage | Sample Buffer (4X) containing lithium dodecyl sulfate (LDS) at pH 8.5 with SERVA Blue G250 and phenol red. Store at 4°C to 25°C. |
Gel Type | Bis-Tris Plus |
Product Type | LDS Sample Buffer |
For Use With (Equipment) | Bolt Mini Gel Tank |
Concentration | 4X |
Gel Compatibility | Bolt™ Bis-Tris Plus Gels |
Product Line | Bolt™ |
A solution used to solubilize native protein samples prior to use with the NativePAGE Gel System
Ready-to-use 5X SDS-PAGE sample loading buffers available in reducing and nonreducing formulations, both with a pink dye buffer-front marker.
Optimized for the transfer of proteins from Bolt Bis-Tris Plus gels to membranes for western blotting
Buffer | Transfer Buffers |
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Content And Storage | Store at 4 to 25°C. |
Form | Liquid |
Product Type | Western Blot Transfer Buffer |
Concentration | 20 X |
Gel Compatibility | Bolt™ Bis-Tris Plus Gels |
Product Line | Bolt™ |
Used to make the NativePAGE Cathode and Anode Running Buffers
Nonreducing lithium dodecyl sulfate sample loading buffer, a unique alternative to homemade and other commercial gel-loading dyes.
Used to prepare protein samples for denaturing gel electrophoresis with NuPAGE™ Novex™ gels. Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X) contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.
Shipping Condition | Approved for shipment at Room Temperature or on Wet Ice |
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Buffer | Sample Loading Buffers |
Content And Storage | Sample Buffer (4X) containing lithium dodecyl sulfate (LDS) at pH 8.5 with SERVA Blue G250 and phenol red. Store at 4°C to 25°C. |
Gel Type | NuPAGE |
Product Type | LDS Sample Buffer |
Gel Compatibility | NuPAGE™ Gels |
Product Line | NuPAGE™ |
Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis
Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats
For Use With (Equipment) | Bolt |
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Concentration | 20X |
Product Line | Bolt |
Formulated for separation of proteins, in their denatured state, on NuPAGE Novex Tris-Acetate gels
Optimized for use with Bolt™ Bis-Tris Plus gels, and available in a variety of formats.
Content And Storage | Sample Reducing Agent (10X) contains 500 mM dithiothreitol (DTT). Store at 2°C to 8°C. |
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Form | Liquid |
Product Type | Sample Reducing Agent |
For Use With (Equipment) | Bolt Mini Gel Tank |
Formulation | 500 mM dithiothreitol (DTT) |
Reagent Type | Reducing Agent |
Concentration | 10X, 500 mM |
Gel Compatibility | Bolt™ Bis-Tris Plus Gels |
Product Line | Bolt™ |
Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting
Shipping Condition | Room Temperature |
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Buffer | Transfer Buffers |
Content And Storage | Store at room temperature. Guaranteed stable for 6 months unless otherwise specified in the product literature. |
Gel Type | NuPAGE |
Product Type | Transfer Buffer |
Shelf Life | 6 Months |
Gel Compatibility | NuPAGE™ Gels |
Product Line | NuPAGE™ |
Digitonin (5%), or digitin, is a non-ionic detergent with a cholesterol-like structure that can water-solubilize lipids, permeabilize cells, be used as a cytotoxicity positive control and solubilize membrane-bound proteins for NativePAGE™ protein analysis or cryo-EM structural analysis.
An optimized pre-mixed IEF cathod buffer