PepMap Columns

banner_new_product_pepmap

The standard for Peptide Separations in Proteomics


acclaim_pepmap_column

Thermo Scientific™Acclaim™ PepMap™ and Acclaim™ PepMap™ RSLC columns are specially designed for high-resolution analyses of tryptic, natural, and synthetic peptides. The columns are often applied for LC-MS/MS peptide mapping for protein identification, biomarker discovery, and systems biology. Due to their high loading capacity, the columns are exceptionally suitable for the analysis of low abundant peptides in complex proteomics samples.

PepMap columns are available in separation (analytical) and trap formats.

Key features & benefits

  • High resolution protein identification for biomarker discovery, verification or any other analysis
  • Highest sensitivity in LC-MS
  • Higher throughput due to higher loading pressure
  • Thermo Scientific™ nanoViper™ fingertight connections for easy column installation

PepMap columns employ nanoViper zero-dead-volume (ZDV) fingertight connections that eliminate the assembly of PEEK sleeve connections making it far easier to achieve state-of-the-art nanospray performance and are capable of withstanding pressures up to 1200 bar.

 

Separation columns
acclaim_pepmap_column

Separation columns come in diameters for nano (50 and 75 ÎĽm), capillary (300 ÎĽm) and micro (1000 ÎĽm) LC. Different lengths are available from 5cm for high throughput with short micro columns to 75 cm long nano columns for high peak capacity and sensitivity.

Narrow Results

Narrow Results

  • (13)
  • (3)
  • (2)
  • (2)
  • (5)
  • (2)
  • (2)
  • (2)
  • (5)
  • (2)
  • (5)
  • (1)
  • (7)
  • (6)
  • (13)
  • (13)
  • (1)
  • (8)
  • (4)
  • (9)
  • (1)
  • (2)
  • (12)
Trap columns
nanotrap

The Acclaim® PepMap100 Nano-Trap columns are available in 100 μm and 200 μm i.d. fused silica, and are packed with 5 μm particle size and 300 Å pore size.

The nano trap design features a single capillary with the trap column at the end and is ideal for high resolution separation of relatively clean samples or samples with hydrophilic peptides.

The cartridge based trap columns have a wider ID and are ideal for large sample amounts or when sample quality demands more robustness.

    Catalogue No. Description Price Stock Quantity  
Trap cartridges
MCA3

Acclaim® PepMap™ μ-Precolumns are very short microcolumns–available in 5 and 15 mm lengths–consisting of a cartridge holder and a set of disposable cartridges. μ-Precolumns are used for sample pre-concentration, sample cleanup, or in various column-switching techniques.

    Catalogue No. Description Price Stock Quantity  
success
11362113 Details
X5 HPLC COLUMN DIONEX PEPMAP(TM) 3196.68 DKK
Estimated Shipment: 09-04-2025
to see stock.
This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
  
Product Specifications
Carbon Load 15%
Length (Metric) 5 mm
Pore Size 100 Ă…
Type Trap Cartridge
Particle Size 5 ÎĽm
For Use With UltiMate 3000 Proteomics MDLC System
Material Stainless Steel
Temperature 60°C
USP Type L1
Column Type Reversed Phase
Packing Material Spherical, Fully Porous, Ultrapure Silica
Diameter (Metric) 0.3 mm
Product Line nanoLC
Stationary Phase C18
Column Format Trap Cartridge
Max. Pressure 7250 psi (500 bar)
Surface Area 450 m²/g
Phase Reversed Phase
Endcapped Yes
pH 2 to 8
13264509 Details
PEPMAP TRAP HOLDER 5MM 30UM NANOVIPER 3555.72 DKK
Estimated Shipment: 07-04-2025
to see stock.
This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
  
Product Specifications
Product Line nanoLC
Length (Metric) 5 mm
Diameter (Metric) 30 ÎĽm (fitting)
Column Type Trap Column Cartridge Holder
Resources