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Invitrogen™ ELISA Stop Solution

A ready-to-use solution intended for use as a stop solution in ELISA applications that use horseradish peroxidase and TMB substrate solution.

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ELISA Substrates and Reagents

Shipping Condition	Ambient
Content And Storage	Store at 2°C to 8°C
Form	Liquid
Product Type	ELISA Stop Solution
Substrate Type	HRP, TMB

Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

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Specifications

ELISA Substrates and Reagents

Substrate Properties	Chemical Substrate
Product Type	ELISA Substrate
Substrate Type	HRP (Horseradish Peroxidase)
Detection Method	Colorimetric

Invitrogen™ Human IL-6 ELISA Kit

ELISA

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Specifications

Life Science Research ELISA Kits

Immunoassay Kit Format	Colorimetric ELISA (TMB substrate) with Plates
Description	Human IL-6 ELISA Kit
Precision	Intra-assay CV <10%; inter-assay CV <10%

Invitrogen™ ELISA Buffer Kit

Concentrated

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ELISA Substrates and Reagents

Invitrogen™ Human IL-18 ELISA Kit

ELISA

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Specifications

Life Science Research ELISA Kits

Target Species	Human
Conjugate	HRP
Interassay CV	8.1%
Intraassay CV	6.5
Gene Symbol	IGIF,IL-18,IL-1 g,IL1F4
Total Assay Time	3 hrs 10 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	9.0 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	78 to 5,000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 20 mins
Accession Number	Q14116
Regulatory Status	RUO
Target	IL-18
Kit Contents	Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Controls,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3606
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Interleukin-18, Interferon-gamma-inducing factor

Invitrogen™ Rat BDNF ELISA Kit

ELISA

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Specifications

Life Science Research ELISA Kits

Protein Family	Growth Factors & Receptors
Host Species	Rat
Conjugate	HRP
Description	BDNF ELISA Kit, 96 Assays, 1 Kit, Rat Species, HRP Conjugate, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of BDNF in Samples Using a Microplate Reader
Intraassay CV	<10
Detectable Analytes	Rat and human BDNF
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 10 μL, Serum 10 μL, Supernatant 100 μL
Assay Sensitivity	12 pg/mL
Research Discipline	Neurobiology
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hrs 20 mins
Detection Method	Colorimetric
Assay	Solid-phase sandwich ELISA
Target	BDNF
Specificity	This ELISA pair antibody detects rat and human BDNF. Other species not determined.
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Label or Dye	HRP
Sufficient For	96 Assays
Calibration Range	12 pg/mL to 3,000 pg/mL
Interassay CV	<12%
Gene Symbol	Bdnf
Storage Requirements	2°C to 8°C
Assay Range	12.29 to 3000 pg/mL
Accession Number	P23363
Regulatory Status	RUO
Product Type	ELISA
Gene ID (Entrez)	24225
Sample Type	Plasma,Serum,Supernatant

Invitrogen™ Mouse DPPIV (DPP4/CD26) ELISA Kit

ELISA

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Specifications

Life Science Research ELISA Kits

Protein Family	Proteases, Peptidases & Inhibitors
Host Species	Mouse
Target Species	Mouse
Conjugate	HRP
Description	DPPIV (DPP4) ELISA Kit, 96 Assays, 1 Kit, Mouse Species, Proteases, Peptidases and Inhibitors Protein Family, Cell Cycle and Proliferation Research Discipline, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Dppiv (Dpp4) i
Intraassay CV	<10
Detectable Analytes	DPPIV (DPP4)
Substrate Type	TMB
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 5 μL, Serum 5 μL, Supernatant 100 μL
Assay Sensitivity	0.45 ng/mL
Research Discipline	Cell Cycle & Proliferation
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hrs 20 mins
Detection Method	Colorimetric
Assay	Solid-phase sandwich ELISA
Target	DPP4
Specificity	This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Label or Dye	HRP
Calibration Range	0.45 ng/mL to 100 ng/mL
Interassay CV	<12%
Gene Symbol	Cd26,Dpp-4,THAM
Storage Requirements	2°C to 8°C
Assay Range	0.41 to 100 ng/mL
Accession Number	P28843
Regulatory Status	RUO
Product Type	ELISA
Gene ID (Entrez)	13482
Sample Type	Plasma,Serum,Supernatant
Synonym	RP23-87G8.2, Cd26, Dpp-4, THAM

Invitrogen™ Human Cortisol Competitive ELISA Kit

ELISA

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Specifications

Life Science Research ELISA Kits

Target Species	Human
Conjugate	HRP
Description	Cortisol Competitive ELISA Kit
Interassay CV	8.1%
Intraassay CV	8.8
Total Assay Time	1 hrs 30 mins
Sample Volume	Fecal Extract 50 μL, Plasma 1 μL, Saliva 12.5 μL, Serum 1 μL, Supernatant 50 μL, Urine 6.25 μL
Assay Sensitivity	17.3 pg/mL
Storage Requirements	-20°C
Research Discipline	Inflammation
Assay Range	100 to 3200 pg/mL
For Use With (Application)	ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
Test Time	1 hrs 20 mins
Detection Method	Fluorescence
Regulatory Status	RUO
Target	Cortisol
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,HRP-Conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Sample Type	Fecal Extract,Plasma,Saliva,Serum,Supernatant,Urine
Label or Dye	HRP

Invitrogen™ Progesterone Competitive ELISA Kit

ELISA

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Specifications

Life Science Research ELISA Kits

Target Species	Human
Conjugate	HRP
Description	Progesterone Competitive ELISA Kit
Interassay CV	5.7%
Intraassay CV	3.9
Total Assay Time	2 hrs 30 mins
Sample Volume	Fecal Extract 50 μL, Supernatant 50 μL, Urine 12.5 μL
Assay Sensitivity	47.9 pg/mL
Storage Requirements	-20°C
Research Discipline	Reproductive Biology
Assay Range	50 to 3200 pg/mL
For Use With (Application)	ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
Test Time	1 hrs 20 mins
Detection Method	Fluorescence
Regulatory Status	RUO
Target	Progesterone
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,HRP-Conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Sample Type	Fecal Extract,Supernatant,Urine
Label or Dye	HRP

Invitrogen™ Rat VEGFR1 ELISA Kit

ELISA

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Specifications

Life Science Research ELISA Kits

Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10
Gene Symbol	FLT1
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	0.41 ng/mL
Storage Requirements	2°C to 8°C
Assay Range	0.41 to 100 ng/mL
Test Time	1 hrs 20 mins
Accession Number	AI323757; Embryonic receptor kinase 2; Emrk2; FLT; Flt1; FLT-1; fms related tyrosine kinase 1; FMS-like tyrosine kinase 1; FMS-related tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor); FRT; i21; sFlt1; soluble VEGFR 1; sVEGFR 1; Tyrosine-protein kinase FRT; tyrosine-protein kinase receptor FLT; Vascular endothelial growth factor receptor 1; vascular endothelial growth factor receptor-1; vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1
Regulatory Status	RUO
Target	VEGF Receptor 1
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	54251
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP

Invitrogen™ Mouse CD36 ELISA Kit

ELISA

Pricing and Availability
Specifications

Life Science Research ELISA Kits

Protein Family	CD Proteins
Host Species	Mouse
Target Species	Mouse
Conjugate	HRP
Description	CD36 ELISA Kit, 96 Assays, 1 Kit, Mouse Species, CD Proteins Protein Family, Apoptosis Research Discipline, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Cd36 in Samples, Using a Microplate Reader
Intraassay CV	<10
Detectable Analytes	CD36
Substrate Type	TMB
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 33 μL, Serum 33 μL, Supernatant 100 μL
Assay Sensitivity	30 pg/mL
Research Discipline	Apoptosis
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hrs 20 mins
Detection Method	Colorimetric
Assay	Solid-phase sandwich ELISA
Target	CD36
Specificity	This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Label or Dye	HRP
Interassay CV	<12%
Gene Symbol	FAT,GPIV,Scarb3
Storage Requirements	2°C to 8°C
Assay Range	27.43 to 20,000 pg/mL
Accession Number	Q08857
Regulatory Status	RUO
Product Type	ELISA
Gene ID (Entrez)	12491
Sample Type	Plasma,Serum,Supernatant
Synonym	FAT, GPIV, Scarb3

Invitrogen™ Mouse HGF ELISA Kit

ELISA

Pricing and Availability
Specifications

Life Science Research ELISA Kits

Protein Family	Chemokine & Receptors
Host Species	Mouse
Target Species	Mouse
Conjugate	HRP
Description	HGF ELISA Kit, 96 Assays, 1 Kit, Mouse Species, Chemokines and Receptors Protein Family, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of HGF in Samples
Intraassay CV	<10
Detectable Analytes	HGF
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity	400 pg/mL
Research Discipline	Apoptosis
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hrs 20 mins
Detection Method	Colorimetric
Assay	Solid-phase sandwich ELISA
Target	HGF
Specificity	This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Label or Dye	HRP
Calibration Range	400 pg/mL to 60 ng/mL
Interassay CV	<12%
Gene Symbol	C230052L06Rik,HGF/SF,NK1,NK2,SF,SF/HGF
Storage Requirements	2°C to 8°C
Assay Range	0.614 to 60 ng/mL
Accession Number	Q08048
Regulatory Status	RUO
Product Type	ELISA
Gene ID (Entrez)	15234
Sample Type	Plasma,Serum,Supernatant
Synonym	C230052L06Rik, HGF/SF, NK1, NK2, SF, SF/HGF

Invitrogen™ TMB ELISA Buffer Kit, PeproTech®

This kit contains the key components required for the analysis of 10 Sandwich ELISA plates. It has been specifically formulated to be used in conjunction with PeproTech’s Standard TMB ELISA Development Kits.

Promotions are available

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ELISA Substrates and Reagents

Invitrogen™ Mouse BLC/CXCL13 ELISA Kit

ELISA

Pricing and Availability
Specifications

Life Science Research ELISA Kits

Protein Family	Chemokine & Receptors
Host Species	Mouse
Target Species	Mouse
Conjugate	HRP
Description	BLC (CXCL13) ELISA Kit, 96 Assays, 1 Kit, Mouse Species, HRP Conjugate, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of BLC (CXCL13) in Samples Using a Microplate Reader
Intraassay CV	<10
Detectable Analytes	BLC (CXCL13), mouse BLC
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 5 μL, Serum 5 μL, Supernatant 100 μL
Assay Sensitivity	2 pg/mL
Research Discipline	Inflammation
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hrs 20 mins
Detection Method	Colorimetric
Assay	Solid-phase sandwich ELISA
Target	CXCL13
Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL- 4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Label or Dye	HRP
Calibration Range	2 pg/mL to 2000 pg/mL
Interassay CV	<12%
Gene Symbol	Cxcl13
Storage Requirements	2°C to 8°C
Assay Range	1.37 to 1000 pg/mL
Accession Number	O55038
Regulatory Status	RUO
Product Type	ELISA
Gene ID (Entrez)	55985
Sample Type	Plasma,Serum,Supernatant
Synonym	4631412M08Rik, ANGIE2, Angie, BCA-1, BLC, BLR1L, Scyb13

Invitrogen™ Mouse CTLA-4 ELISA Kit

ELISA

Pricing and Availability
Specifications

Life Science Research ELISA Kits

Protein Family	Chemokine & Receptors
Host Species	Mouse
Target Species	Mouse
Conjugate	HRP
Intraassay CV	<10
Detectable Analytes	CTLA-4
Substrate Type	TMB
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity	5 pg/mL
Research Discipline	Immunology
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hrs 20 mins
Detection Method	Colorimetric
Assay	Solid-phase sandwich ELISA
Target	CTLA-4 (CD152)
Specificity	This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL- 17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Label or Dye	HRP
Calibration Range	5 pg/mL to 1200 pg/mL
Interassay CV	<12%
Gene Symbol	Cd152,Ctla-4,Ly-56
Storage Requirements	2°C to 8°C
Assay Range	4.92 to 1200 pg/mL
Accession Number	P09793
Regulatory Status	RUO
Product Type	ELISA
Gene ID (Entrez)	12477
Sample Type	Plasma,Serum,Supernatant
Synonym	CH29-120A16.2, Cd152, Ctla-4, Ly-56

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Search results for "Elisa reader.html"

Invitrogen™ ELISA Stop Solution

Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Invitrogen™ Human IL-6 ELISA Kit

Invitrogen™ ELISA Buffer Kit

Invitrogen™ Human IL-18 ELISA Kit

Invitrogen™ Rat BDNF ELISA Kit

Invitrogen™ Mouse DPPIV (DPP4/CD26) ELISA Kit

Invitrogen™ Human Cortisol Competitive ELISA Kit

Invitrogen™ Progesterone Competitive ELISA Kit

Invitrogen™ Rat VEGFR1 ELISA Kit

Invitrogen™ Mouse CD36 ELISA Kit

Invitrogen™ Mouse HGF ELISA Kit

Invitrogen™ TMB ELISA Buffer Kit, PeproTech®

Invitrogen™ Mouse BLC/CXCL13 ELISA Kit

Invitrogen™ Mouse CTLA-4 ELISA Kit

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