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Gibco™ AAV-MAX Helper-Free AAV Production System Kit, without lysis buffer
The Gibco™ AAV-MAX Helper-Free AAV Production System is a scalable and high-titer platform for suspension AAV production. It is based on a high-density suspension culture of clonal 293F-derived Viral Production Cells 2.0 in Viral Production Medium.
Brand: Gibco™ A54824
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Description
- A scalable and high-titer platform for suspension AAV production
- Based on a high-density suspension culture of clonal 293F-derived Viral Production Cells 2.0 in Viral Production Medium
- Optimal viral titers are mediated by our advanced lipid nanoparticle transfection reagent in combination with a novel production enhancer
- The components are designed to work synergistically, resulting in maximal titers and eliminating the need to optimize reagents and protocols
- Note, this version of the kit does not contain the AAV-MAX Lysis Buffer
- To purchase the kit including the AAV-MAX Lysis Buffer, please see A51217
- All components are also available for purchase separately
Features of the AAV-MAX Helper-Free AAV Production System include:
- Components work synergistically to produce high AAV titers greater than 5x1010 vg/mL (unpurified)
- Suspension-based system has scalable protocols from shake flask to bioreactor scale
- Simplified workflow—streamlined protocol using helper virus-free triple transfection
- Clonal, 293F-derived, high-producer cell line
- Robust transfection protocols are adaptable to large scale transfection
Components of the AAV-MAX System Kit include:
- 1 vial of Viral Production Cells 2.0
- 1 L of Viral Production Medium
- 1 AAV-MAX Transfection Kit for 1 L of production
- 100 mL Viral-Plex Complexation Buffer
- 100 mL GlutaMAX™ Supplement
Specifications
| • 1 vial Viral Production Cells 2.0 • 1 L Viral Production Medium • 1 AAV-MAX Transfection kit (for 1 L production) • 100 mL Viral-Plex Complexation Buffer • 100 mL GlutaMAX |
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| Kidney (Embryonic) | |
| AAV Production System | |
| AAV production | |
| 1 kit |
| Viral Production Cells 2.0 | |
| Suspension Cell Culture | |
| Sterile-filtered | |
| AAV-MAX |