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Molecular Probes™ AbC™ Anti-Mouse Bead Kit
AbC™ Anti-Mouse Bead Kit
Brand: Molecular Probes™ A10344
This item is not returnable.
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Description
The AbC™ Anti-Mouse Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated mouse antibodies with any laser in flow cytometry.Accurate compensation is crucial for effective multicolor analysis in flow cytometry. Often, however, cellular-based compensation controls are not completely effective, as many antigens are not highly expressed, and dimly stained cells can lead to inaccurate compensation settings. The AbC™ Anti-Mouse Bead Kit was developed to address this issue.
How it works
Each kit provides polystyrene microspheres that have fluorescent antibody conjugate capture capacity (positive compensation beads) or are inert (negative compensation beads). After mixing with a fluorophore-conjugated mouse antibody, the two components provide a distinct high-signal positive control with an appropriate negative population that can then be used to set compensation properly regardless of the intensity of the cells in the actual experiment.
Species reactivity
The AbC™ Anti-Mouse Bead Kit is ideal for compensation with all isotypes of mouse (IgG1, IgG2a, IgG2b, IgG3, IgM) immunoglobulins.
Maximum laser compatibility
All AbC™ compensation bead kits are ideal for use with any excitation source, including the 405 nm laser which has historically provided higher background signals due to auto-fluorescence with other kits on the market.
Specifications
| Contains 1 vial AbC™ capture beads and 1 vial of negative beads. |
|
| 6 μm | |
| Flow Cytometer | |
| 100 tests | |
| Room Temperature | |
| CompenFlow™, Molecular Probes™ |
| Fluorescence | |
| any lasers (UV to 633/635) | |
| Tube | |
| 1 kit | |
| Surface | |
| Compensation Beads |
For Research Use Only. Not for use in diagnostic procedures.