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Invitrogen™ Amino Allyl MessageAmp™ II aRNA Amplification Kit
Provides reagents for aRNA amplification with Amino allyl NTP incorporation
Brand: Invitrogen™ AM1753
Includes: 60μL T7 Oligo(dT) Primer; 22μL ArrayScript™ Reverse Transcriptase; 22μL RNase Inhibitor; 42μL 10X First Strand Buffer; 170μL dNTP Mix; 210μL 10X Second Strand Buffer; 42μL DNA Polymerase; 22μL RNase H; 84μL T7 Enzyme Mix; 84μL 10X Reaction Buffer; 95μL 50mM UTP Solution; 64μL 50mM 5-(3-amino allyl)-UTP; 255μL ATP, CTP, GTP Mix; 40μL Second Round Primers; 10μL 1mg/mL Control RNA; 1.75mL Nuclease-free Water; 400μL Coupling Buffer; 440μL DMSO; 180μL 4M Hydroxylamine; 40mL Wash buffer; 7mL cDNA Binding Buffer; 20mL aRNA Binding Buffer; 20 aRNA Filter Cartridges; 40 aRNA Collection Tubes; 20 cDNA Filter Cartridges and Tubes; 20 cDNA Elution Tubes; 10mL Nuclease-free Water; 20 Labeled aRNA Filter Cartridges and Tubes; 20 Labeled aRNA Elution Tubes
Description
Improved first- and second-strand cDNA synthesis
Included in the kit is ArrayScript™ RT, a rationally engineered M-MLV reverse transcriptase, which produces equivalent or higher yields of full-length cDNA compared to other enzymes. The second-strand cDNA synthesis reaction has also been optimized to be compatible with the first-strand cDNA products generated with ArrayScript RT to allow maximal conversion of first-strand cDNA into full-length double-stranded cDNA templates.
Increased labeling efficiency of your aRNA
The Amino Allyl MessageAmp II aRNA Amplification Kit incorporates amino allyl NTP into the aRNA followed by the coupling of its reactive amino group to an NHS ester label (e.g., Cy or another dye). This strategy offers several advantages over the direct incorporation of labeled NTPs. Direct incorporation of labeled NTPs is inefficient and results in low yields and low specific activity aRNA and high costs. Unlike lableled NTPs, amino allyl–modified NTPs are incorporated almost as efficiently as unmodified NTPs and are much less expensive than the dye coupled NTPs.
- Kit includes sufficient reagents for 20 reactions
- Cy™ dyes are not included
Expression Array Labeling, Gene Expression Analysis and Genotyping, Microarray Analysis, RNA Amplification
Specifications
| aRNA Amplification Kit | |
| No | |
| ArrayScript™ | |
| Kit | |
| 20 Reactions |
| • 60 μl T7 Oligo(dT) Primer (-20°C) • 22 μl ArrayScript™ Reverse Transcriptase (-20°C) • 22 μl RNase Inhibitor (-20°C) • 42 μl 10X First Strand Buffer (-20°C) • 170 μl dNTP Mix (-20°C) • 210 μl 10X Second Strand Buffer (-20°C) • 42 μl DNA Polymerase (-20°C) • 22 μl RNase H (-20°C) • 84 μl T7 Enzyme Mix (-20°C) • 84 μl 10X Reaction Buffer (-20°C) • 95 μl 50 mM UTP Solution (-20°C) • 64 μl 50 mM 5-(3-amino allyl)-UTP (-20°C) • 255 μl ATP, CTP, GTP Mix (-20°C) • 40 μl Second Round Primers (-20°C) • 10 μl 1 mg/ml Control RNA (-20°C) • 1.75 ml Nuclease-free Water (any temperature) • 400 μl Coupling Buffer (-20°C) • 440 μl DMSO (-20°C) • 180 μl 4M Hydroxylamine (-20°C) • 40 ml Wash buffer (4°C or room temperature) • 7 ml cDNA Binding Buffer (room temperature) • 20 ml aRNA Binding Buffer (room temperature) • 20 aRNA Filter Cartridges (room temperature) • 40 aRNA Collection Tubes (room temperature) • 20 cDNA Filter Cartridges + Tubes (room temperature) • 20 cDNA Elution Tubes (room temperature) • 10 ml Nuclease-free Water (any temperature) • 20 Labeled aRNA Filter Cartridges + Tubes (room temperature) • 20 Labeled aRNA Elution Tubes (room temperature) |
|
| Indirect Labeling | |
| Poly(A+) RNA, Total RNA | |
| Ambion™, MessageAmp™ |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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