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Cytiva Amersham™ ECL™ Direct Nucleic Acid Labeling/Detection System
Use for fast and easy detection of medium-to-high-target amounts. Cytiva Life Sciences™ Amersham™ ECL™ Direct Nucleic Acid Labeling/Detection System are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction
Brand: Cytiva RPN3001
Includes: 45-000-922 and 45-000-923: Labeling reagent; Crosslinker; Control DNA; Blocking agent; ECL detection reagents and ECL Gold hybridization buffer
Description
- Direct probe labeling in a 10 min reaction, 1 h from hybridization to detection with ECL Direct, Hybond N+, and Hyperfilm ECL.
- Eliminates handling, waste, and regulatory issues associated with the use of radioactivity.
- No need to strip blots before reprobing.
- For fast and easy detection of medium- to high-target amounts in applications such as colony/plaque screens, dot blots, and PCR product analyses.
- Consistent results combining strong signals with very low backgrounds.
Specifications
| 2°C to 8°C | |
| DNA labeling reagent (2 x 0.5mL); Glutaraldehyde solution (2 x 0.5mL); Water (2 x 1.0mL); l Hind III control DNA 10μg/mL (2 x 0.1mL); Gold hybridization buffer (2 x 500mL); Blocking agent (2 x 25g); ECL detection reagent 1 (2 x 125mL); ECL detection reagent 2 (2 x 125mL) | |
| For Labeling 10μg Nucleic Acid |
| Colony or plaque screens, dot blots and PCR product analyses | |
| ECL Direct Labeling and Detection System | |
| 20 min. |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Danger
Hazard Category
- Serious eye damage/eye irritation Category 2
- ACUTE AQUATIC Acute 1
- Skin corrosion/irritation Category 2
- Serious eye damage/eye irritation Category 2
- Skin sensitiser Category 1
- Respiratory sensitiser Category 1
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
- LONG-TERM AQUATIC HAZARD Chronic 3
- Skin sensitiser Category 1
- Specific target organ toxicity Category 3
Hazard Statement
- H319-Causes serious eye irritation.
- H315-Causes skin irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H317-May cause an allergic skin reaction.
- H335-May cause respiratory irritation.
- H412-Harmful to aquatic life with long lasting effects.
- H400-Very toxic to aquatic life.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P273-Avoid release to the environment.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P391-Collect spillage.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- P501-Dispose of contents/container to .
Supplemental information
- MIXTURE LIST-Contains : glutaral, urea, sodium dodecyl sulphate