AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.
Brand: Applied Biosystems™ 4317742
85204.26 DKK valid until 2025-03-29
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AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation. This modified enzyme is provided in an inactive state and upon thermal activation the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.
Features
AmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre-PCR heat step or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold DNA Polymerase is a chemical hot-start enzyme, there is limited risk of biological contamination.
GeneAmp 10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold DNA Polymerase, resulting in a highly specific, robust PCR amplification. The ionic strength and pH of GeneAmp 10X PCR Gold Buffer have been optimized to provide a wider activation temperature and time range when used in conjunction with AmpliTaq Gold DNA Polymerase.
Notes
Shipping condition: Dry Ice
| This package includes 5 boxes, each box contains: • AmpliTaq Gold DNA Polymerase (5 U/μL), 5 x 200 μL • GeneAmp 10X Gold Buffer, 20 x 1.5 mL • 25 mM MgCl2, 20 x 1.5 mL Store at -15°C to -30°C. |
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| Low | |
| Standard | |
| DNA Polymerase | |
| Dry Ice | |
| 5 U/μL | |
| Built-In Hot Start | |
| 3'-A | |
| 5 kb or less |
| Tube | |
| AmpliTaq Gold DNA Polymerase | |
| 5' - 3' | |
| 25,000 Units | |
| Hot-start PCR | |
| 1X | |
| 20,000 Reactions | |
| Separate Components | |
| DNA |
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For Research Use Only. Not for use in diagnostic procedures.
