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Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix

AmpliTaq Gold 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package–all components are premixed and premeasured.

Brand: Applied Biosystems™ 4398901

16907.51 DKK valid until 2025-03-29
Use promo code "24111" to get your promotional price.

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Product Code. 10219584

25360.00 DKK / Pack of 10

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Includes: AmpliTaq Gold 360 Master Mix is supplied at 2X concentration and the 360 GC Enhancer. Typically, 1.5mL of the 360 GC Enhancer is supplied with each 5mL of master Mix.. Source: Recombinant, modified form of theThermus aquaticusDNA polymerase gene expressed in E. coli. All solutions are prepared in deionized, filter-sterilized water.

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Description

AmpliTaq Gold 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package with all components premixed and premeasured. Designed for convenience, this master mix scales to various reaction volumes for greater application and format flexibility. The main ingredient of the master mix is AmpliTaq Gold 360 DNA Polymerase, but it also contains the world-class 360 GC Enhancer, which can be added for high GC-content templates. With the wide template coverage, optimization of PCR reaction conditions is virtually eliminated with the use of AmpliTaq Gold 360 Master Mix.

Features

Optimized for a broad range of targets
High sensitivity, specificity, and yield
Robust amplification of GC-rich sequences with market-leading 360 GC Enhancer
Helps achieves high-quality sequencing data
Easy-to-use, premixed master mix

Optimized for easy and challenging targets

Challenging targets include AT-rich, GC-rich, primer-dimer forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can now be reproducibly amplified with a single reagent under standardized conditions. Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq Gold 360 as a high-performing enzyme, ensuring the highest probability of success for the amplification of both everyday and challenging targets.

Optimized for automated, hot-start PCR

AmpliTaq Gold 360 DNA Polymerase is the key ingredient in an automated, convenient, and efficient hot-start PCR. When AmpliTaq Gold 360 PCR Master Mix is added to the reaction mixture at room temperature, the inactive enzyme is not capable of primer extension. Any low-stringency mispriming events that may have occurred will not be enzymatically extended and subsequently amplified. An initial thermal incubation step is required for activation and ensures that active enzyme is generated only at temperatures where the DNA is fully denatured. The amount of AmpliTaq Gold 360 DNA Polymerase increases in the reaction slowly with each cycle number, and specific product yield increases without buildup of nonspecific products, including primer dimers. The extreme specificity allows easier multiplexing and allelic discrimination.

Amplify low-copy amplicons and long targets

AmpliTaq Gold 360 DNA Polymerase efficiently amplifies targets present at low copy number (see figure), even in the presence of high concentrations of complex DNA, making it especially suited for low-copy pathogen detection and amplification of targets from degraded DNA samples. AmpliTaq Gold 360 DNA Polymerase efficiently and reproducibly amplifies long (up to 5 kb) sequences.

Notes

AmpliTaq Gold 360 PCR Master Mix is supplied at 2X the recommended usage concentration for easy dilution when adding template and primers.

Order Info

Approved for shipment at Room Temperature or on Wet Ice

Specifications

Content And Storage	• 2X AmpliTaq Gold 360 Master Mix, 10 x 5 mL • 360 GC Enhancer, 10 x 1.5 mL Store at -20°C until first use, then store at 4°C.
GC-Rich PCR Performance	High
Reaction Speed	Standard
Green Features	Sustainable packaging
Quantity	10 x 5 mL
For Use With (Application)	Cloning, Hot-start PCR
Fidelity (vs. Taq)	1X
No. of Reactions	2000 Reactions
Reaction Format	SuperMix or Master Mix
Starting Material	DNA

Format	Tube
Polymerase	AmpliTaq Gold 360 DNA Polymerase
Exonuclease Activity	5' - 3'
Product Type	PCR Master Mix
Shipping Condition	Room Temp or Wet Ice
Concentration	2X
Hot Start	Built-In Hot Start
Overhang	3'-A
Size (Final Product)	5 kb or less
Volume	50 mL

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Documents

Scientific Resources

Brochures

AmpliTaq® 360 Polymerases - Green Fact Sheet File size: 347.2 kB

Brochures & Specifications: AmpliTaq Gold® 360 Master Mix: AmpliTaq Gold® 360 DNA Polymerase File size: 1.7 MB

Quick Reference Card: AmpliTaq Gold® 360 Master Mix File size: 106.2 kB

Amplifi cation of Long GC-Rich Sequences File size: 1.1 MB

Brochures & Specifications: 360 GC Enhancer: Use of the 360 GC Enhancer for Optimal PCR File size: 1.2 MB

Application Notes

Application Notes & Tutorials: Amplification of Long GC-Rich Sequences: Applied Biosystems AmpliTaq Gold® 360 Master Mix File size: 1.1 MB

Product Information

Manuals

Product Bulletin: AmpliTaq Gold 360 Master Mix and 360 DNA Polymerase File size: 1.7 MB

Protocol: AmpliTaq Gold 360 Master Mix Version: MAN0009869 Rev A (06Aug2014) File size: 968.8 kB

Protocol: AmpliTaq Gold® 360 Master Mix (English ) Version: 20 Jul 2010 File size: 343.7 kB

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