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Applied Biosystems™ TaqMan™ Reverse Transcription Reagents

Marca: Applied Biosystems™ 4304134

37170.00 DKK valid until 2025-06-30
Use promo code "24108" to get your promotional price.

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Código de producto. 10279624

53100.00 DKK / 1 set

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Includes: MultiScribe™ Reverse Transcriptase (100μL at 50U/μL)

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Descripción

TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)₁₆, and reverse primers are included for cDNA synthesis flexibility. These reagents are designed for reverse transcription and PCR amplification of a specific target RNA from either total RNA or mRNA. The reagents in this kit facilitate two-step RT-PCR, which is performed in two separate reactions and is useful when detecting multiple transcripts from a single cDNA reaction or when storing a portion of the cDNA.

Advantages of TaqMan Reverse Transcription Reagents

Contains MultiScribe Reverse Transcriptase, a recombinant Moloney murine leukemia virus (MMLV) reverse transcriptase
Includes random hexamers and oligo (dT)₁₆ for flexibility
Individual components provide versatility in assay setup
Compatible with two-step RT-PCR reactions

Using TaqMan Reverse Transcription Reagents

TaqMan Reverse Transcription Reagents provide all the necessary components for reverse transcription of RNA to cDNA
For two-step RT-PCR, random hexamers, oligo (dT)₁₆, and sequence-specific reverse primers can all be used
The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems

Especificaciones

Content And Storage	Includes 10 units. Each unit contains: • MultiScribe Reverse Transcriptase, 100 μL (50 U/μL) • RNase Inhibitor, 100 μL (20 U/μL) • dNTP Mixture, 1 mL (2.5 mM each dNTP) • Oligo (dT)₁₆, 100 μL (50 μM) • Random Hexamers, 100 μL (50 μM) • 10X RT Buffer, 1.5 mL • MgCl₂ Solution, 1.5 mL Store at –25°C to –15°C.
Technique	Reverse Transcription
Quantity	2000 Reactions
Ribonuclease H Activity	Yes
For Use With (Application)	Real Time PCR (qPCR)
Final Product Type	First-Strand cDNA
Reaction Format	Separate components
Size (Final Product)	Up to 5 kb

Format	Kit
Optimal Reaction Temperature	42°C
Reverse Transcriptase	MultiScribe
Shipping Condition	Dry Ice
Concentration	50 U/μL
No. of Reactions	2000 Reactions at 10 μL
Reagent Type	Reverse Transcription
Starting Material	RNA

Sugerencias de productos Documentos

Documentos

Scientific Resources

Application Notes

Application Tutorial: Applied Biosystems Real-Time PCR Rapid Assay Development Guidelines File size: 243.7 kB

Brochures

Essentials of Real Time PCR File size: 153.6 kB

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