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BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit with BD GolgiStop™
Provides GolgiStop protein transport inhibitor containing monensin
Brand: BD 554715
Includes:
- Fixation/Permeabilization solution (125mL)
- BD Perm/Wash™ Buffer, 10x concentrate containing Fetal Bovine Serum (FBS) and saponin (100mL)
- Protein transport inhibitor BD GolgiStop™ (containing Monensin)
Description
The ex vivo addition of GolgiStop to in vitro- or in vivo -stimulated cells blocks their intracellular transport processes. This results in the accumulation of most cytokine proteins in the Golgi complex and thereby enhances cytokine staining signals.
- GolgiStop reagent is provided for treating up to 1L of cell culture at a cell density of up to 2 × 106 cells/mL
Specifications
| 4°C | |
| 250 | |
| Fixation/Permeabilization Reagent |
| 125 mL fixation/permeabilization solution, perm/Wash™ buffer, fetal bovine serum (FBS) 10X and 100 mL saponin, protein transport inhibitor BD GolgiStop™ | |
| RUO |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- BD Cytofix/Cytoperm Plus Fixation/Permeabilization Kit with Golgistop
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 4
- Carcinogenicity Category 1A
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 2
- Germ cell mutagenicity Category 2
- Skin corrosion/irritation Category 2
- Skin sensitiser Category 1
- Specific target organ toxicity Category 3
Hazard Statement
- H225-Highly flammable liquid and vapour.
- H315-Causes skin irritation.
- H317-May cause an allergic skin reaction.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H335-May cause respiratory irritation.
- H341-Suspected of causing genetic defects.
- H350-May cause cancer.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : ethanol, monensin mononatriumsalz, formaldehyde