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Description
CD DG44 Medium is a chemically defined, protein-free medium optimized for the growth of dihydrofolate reductase deficient (DHFR-) Chinese Hamster Ovary (CHO) cells and expression of recombinant proteins in suspension culture. CD DG44 fully chemically defined formulation contains no lysates, hydrolysates, nor proteins or peptide components of animal, plant, or synthetic origin.
When to Use CD DG44 Medium
You use parental DG44 or DXB11 CHO cells (dhfr-)
When to Consider Another Gibco™ Medium
Consider CD FortiCHO™ medium when:
The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
Maximum batch culture cell densities and protein titers are needed
You have time to adapt cells into a new medium in an effort to maximize titers
Cell health at harvest is a priority for downstream processing
Consider CD OptiCHO™ medium when:
You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
Your CHO cell line is finicky, i.e. hard to grow or adapt
Consider CD CHO medium when:
Your GS CHO cells are recommended to be grown in CD CHO medium
Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)
Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco™ cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
When to Use CD DG44 Medium
When to Consider Another Gibco™ Medium
Consider CD FortiCHO™ medium when:
Consider CD OptiCHO™ medium when:
Consider CD CHO medium when:
Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco™ cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.

Specifications
Specifications
Cell Line | CHO DG44 |
Classification | Animal Origin-free, Chemically-defined, Protein-free, Serum-free |
Concentration | 1 X |
Form | Liquid |
Product Type | CD DG44 Medium |
Serum Level | Serum-free |
With Additives | Sodium Pyruvate, Sodium Bicarbonate, Thymidine, Hypoxanthine |
Without Additives | No Glutamine, No Phenol Red |
Product Line | Gibco™ |
Quantity | 1 L |
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For Research Use Only. Not for use in diagnostic procedures.
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