missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Molecular Probes™ Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit
Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit
Brand: Molecular Probes™ C10418
3808.00 DKK valid until 2025-03-16
Use promo code "24070" to get your promotional price.
Alert:
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24111” to get your promotional price
Description
The Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. Newly synthesized DNA is analyzed using the 405 nm laser of the flow cytometer.View selection guide for all Click-iT™ EdU and Click-iT™ Plus EdU assays for flow cytometry.
An Advanced Method Giving You Results Superior to BrdU
The most accurate method of proliferation analysis is direct measurement of DNA synthesis. Originally, this was performed through incorporation of radioactive nucleosides, i.e., 3H-thymidine. This method was replaced by antibody-based detection of the nucleoside analog bromodeoxyuridine (BrdU). The Click-iT™ EdU Flow Cytometry Assay Kits are novel alternatives to the BrdU assay. EdU (5-ethynyl-2´-deoxyuridine) is a thymidine analog which is incorporated into DNA during active DNA synthesis. Detection is based on click chemistry: a copper catalyzed covalent reaction between an azide and an alkyne. In this application, the alkyne is found in the ethynyl moiety of EdU, while the azide is coupled to Alexa Fluor ™ 488, Alexa Fluor™ 647, or Pacific Blue™ dyes. Standard flow cytometry methods are used for determining the percentage of S-phase cells in the population (Fig 1).
Mild Conditions Allow Use with Cell Cycle Dyes and Antibodies
The advantages of Click-iT™ EdU labeling are readily evident while performing the assay (Fig 2). The small size of the dye azide allows for efficient detection of the incorporated EdU using mild conditions, while standard aldehyde-based fixation and detergent permeabilization are sufficient for the Click-iT™ detection reagent to gain access to the DNA. This is in contrast to BrdU assays that require DNA denaturation (using HCl, heat, or digestion with DNase) to expose the BrdU so that it may be detected with an anti-BrdU antibody. Sample processing for the BrdU assay can result in signal alteration of the cell cycle distribution as well as destruction of antigen recognition sites when using the HCl method. In contrast, the easy-to-use EdU cell proliferation kit is compatible with cell cycle dyes. This EdU assay kit can also be multiplexed with antibodies against surface and intracellular markers.
Quick and Simple Protocol
The Click-iT™ EdU protocol is based on the aldehyde fixation and detergent permeabilization steps for immunohistochemical antibody labeling, but EdU is compatible with other fixation/permeabilization agents including saponin and methanol. In just five steps you’ll be ready to analyze your cell proliferation data:
Get Accurate Results Economically
By increasing the number of assays per kit, the Click-IT™ EdU Pacific Blue™ Flow Cytometry Assay Kit is less expensive than the traditional BrdU assays making them ideal for large experiments.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Contains EdU (5-ethynyl-2' -deoxyuridine), Pacific Blue™ azide, anhydrous dimethylsulfoxide (DMSO), Click-iT™ fixative, Click-iT™ saponin-based permeabilization and wash buffer, copper (II) sulfate,, and Click-iT™ EdU buffer additive. |
|
| Flow Cytometer | |
| Pacific Blue™ | |
| Tube(s) | |
| Click-iT™, Pacific Blue™ | |
| Room Temperature |
| Fluorescence | |
| Flow Cytometry Assay Kit | |
| 404⁄450 | |
| Less hazardous | |
| 50 Assays |
Product Suggestions
Customers who viewed this item also viewed
Viewing 1-5 of 12
-
Catalog No. 12077549
kr 1.570,00 / 5000mL -
Catalog No. 10678484
kr 1.486,00 / 500mL -
Catalog No. 13464189
kr 768,00 / Pack of 5 -
Catalog No. 10647894
kr 2.050,00 / 250mL -
-
-
Catalog No. 10729174
kr 2.300,00 / 500µL -
Catalog No. 15137105
kr 86.520,00 / Each -
Catalog No. 11514270
kr 6.720,00 / 100µL -
Catalog No. 10161053
kr 2.695,00 / Each -
Catalog No. 12023725
kr 1.630,00 / 25µg -
Catalog No. 10013162
kr 2.235,00 / Each
Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
5 results found
Special Offers
-
Multiple Manufacturers
More Discovery, Less Cost
Save on thousands of eligible products.Promo Code: 24111Expires: 2025-03-21
Days
5Hours
22Minutes
5Seconds
42New Offer Save Now
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
Molecular Probes™ Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit > 50 assays
For Research Use Only. Not for use in diagnostic procedures.
Spot an opportunity for improvement?Share a Content Correction