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Thermo Scientific™ DMEM for SILAC Product Code.: 15645729

Thermo Scientific™ DMEM for SILAC

Product Code. 15645729 Shop All Thermo Scientific Products
500 mL, 500mL
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Quantity:
500 mL
6 x 500 mL
Unit Size:
500mL
Pack of 6
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Product Code. 15645729

Brand: Thermo Scientific™ 88364

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This item is not returnable. View return policy

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

Thermo Scientific™ DMEM for SILAC is optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS).

Features of DMEM for SILAC:

  • Flexible—liquid media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling
  • High quality—medium is sterile, endotoxin-free, and cell culture-compatible

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of 'heavy' 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization, and quantitation of proteins.

This SILAC medium is used together with heavy and light amino acids for the differential isotopic labeling of cells for SILAC analysis. SILAC media supplemented with heavy amino acids are capable of nearly 100% label incorporation into proteins of living cells.

Related products

  • NeuCode™ Lysine -080, 50 mg (A33613)
  • Fetal Bovine Serum, dialyzed, US origin (26400036)
  • RPMI 1640 Media for SILAC (88365)
TRUSTED_SUSTAINABILITY

Specifications

For Use With (Equipment) Mass Spectrometer
Product Type Media
Content And Storage 1 x 500 mL, store at 2–8°C
Label or Dye Mass Isotope of Amino Acid
Shipping Condition Room Temperature
Starting Material Protein Samples
Final Product Type Liquid Medium
Detection Method Mass Spectrometry
Workflow Step Cell Culture
Quantity 500 mL
Form Liquid
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Certificates

A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.

5 results found
Lot Number Certificate Type Date Product Code
Lot NumberZJ4375581 Certificate TypeCertificate of Analysis Date15/10/2024 Product Code88364
Lot NumberZB4114631 Certificate TypeCertificate of Analysis Date17/02/2024 Product Code88364
Lot NumberYJ3982312 Certificate TypeCertificate of Analysis Date08/11/2023 Product Code88364
Lot NumberXK3745521 Certificate TypeCertificate of Analysis Date28/02/2023 Product Code88364
Lot NumberXG3654661 Certificate TypeCertificate of Analysis Date04/11/2022 Product Code88364

For Research Use Only. Not for use in diagnostic procedures.

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