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Gibco™ Expi293™ Inducible Expression System Kit
Expi293 Inducible Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian 293 cells
Brand: Gibco™ A39251
11056.80 DKK valid until 2024-11-30
Use promo code "21651" to get your promotional price.
This item is not returnable.
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Description
- System kit is centered on engineered Expi293F cells that stably express the tetracycline repressor (TetR) protein, thus enabling tetracycline-regulated protein expression
- Inducible cell line was established by the stable integration of the regulatory plasmid pcDNA6/TR, which encodes the TetR gene under the control of the human CMV promoter
- This cell line is a powerful tool for tetracycline-inducible high-yield expression of recombinant proteins
Features:
- Tetracycline-inducible protein expression with extremely low basal expression levels
- Equivalent protein yields to parental Expi293F cells—up to 1 g/L
- pcDNA5/TO expression vector for tetracycline-regulated expression of the gene of interest
- Suspension, high-density culture of Expi293F Inducible cells in Expi293 Expression Medium
- High-efficiency transient transfection using ExpiFectamine 293 reagent in combination with specialized transfection enhancers
Components included:
- 1 vial of frozen Expi293F Inducible cells
- 1 L of Expi293 Expression Medium
- 1 ExpiFectamine 293 transfection kit sufficient to transfect 1 L of culture
- 100 mL Opti-Plex Complexation Buffer
- 1 pcDNA5/TO Mammalian Expression Vector kit
- 500 mg tetracycline hydrochloride
- pRABBIT IgG IRES-EmGFP positive control vector
All components of the Expi293 Inducible Expression System are also available for individual purchase
Specifications
| Inducible Expression System Kit | |
| 1 Kit | |
| Approved for shipment at Room Temperature or on Wet or Dry Ice | |
| Expi293F™ | |
| Protein Expression |
| Transfection | |
| • Expi293F Inducible Cells (1x107 cells/vial), store in vapor-phase liquid nitrogen • 1 L Expi293 Expression Medium, store at 2–8°C (protect from light) • ExpiFectamine 293 Transfection Kit for 1 L of culture, store at 2–8°C • 100 mL Opti-Plex Complexation Buffer, store at 2–8°C (protect from light) • 20 μg pcDNA5/TO Mammalian Expression Vector, store at 2–8°C or long term storage at -20°C. • pRABBIT IgG IRES-EmGFP Positive Control Vector, store at 2–8°C or long term storage at -20°C. • 500 mg Tetracycline Hydrochloride, store at -20°C |
|
| DNA | |
| Expi293™ | |
| CMV/TO |
For Research Use Only. Not for use in diagnostic procedures.