Thermo Scientific™ Extensor Long PCR Enzyme Mix Products

Description

Extensor Long Range PCR Enzyme is a blend of ThermoPrime Taq DNA Polymerase and a proprietary thermostable proofreading enzyme. The two enzymes act synergistically to generate long PCR products, up to 20 kb. Extensor Long Range Enzyme includes 2 buffer formulations; Buffer 1 for templates up to 12 kb in length, and Buffer 2 for templates 12 to 20 kb and GC-rich or other difficult templates. This enzyme blend is also available in 2X Master Mix and ReddyMix formulations. The ReddyMix Master Mix contains an inert red dye and a density reagent that allows for direct loading onto agarose gels.

• Amplifies targets up to 20kb in length
• Unique enzyme blend and optimized buffer combination delivers increased accuracy and yield
• PCR products generated can be used directly in T/A cloning
• Optimized buffering systems for success with a variety of templates
• Buffer 1 for success with most templates up to 12kb in length
• Buffer 2 for templates 12-20kb and GC-rich or other difficult templates of any length

Format Options:

• Enzyme Kits are the most basic format. Kits include the enzyme, PCR buffers 1 and 2, and 25mM MgCl₂ solution, each in separate vials. dNTPs must be purchased separately

• Complete PCR Kits include all the components contained in the Enzyme Kit, plus they have HPLC-purified dNTP mix. Complete PCR kits are a convenient option that provides all the components necessary for PCR

• Master Mix formats consist of a single master buffer containing all the components needed for PCR. Master Mixes are the most convenient format and significantly reduce assay set-up steps

• ReddyMix Master Mix formats consist of a single ReddyMix master buffer containing all the components needed for PCR. ReddyMix master mix can be directly loaded onto agarose gels post-PCR

• PCR Buffers: All kits are supplied with both Buffer 1 and Buffer 2 to accommodate a variety of template types. Master mixes come in either Buffer 1 or Buffer 2 formulation

Recommended for:

Amplifying fragments up to 20kb; PCR; Cloning; Long PCR