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Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotinylation Kit

Biotinylate protein amines (e.g., lysines) with our shortest (13.5A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Brand: Thermo Scientific™ 21425

Product Code. 10321634

3540.00 DKK / 1 set

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This item is not returnable. View return policy

Description

Label antibodies, proteins and other molecules that have primary amines with Thermo Scientific™ EZ-Link Sulfo-NHS-Biotin, a short-chain, water-soluble biotinylation reagent.

Highlights:

Shorter spacer arm than Sulfo-NHS-LC-Biotin
Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate, or phosphate buffers
Negative charge of -SO₃ keeps biotinylation localized at the cell surface so reagent does not pass through cell membrane
Water solubility eliminates the need for harmful organic solvents

U.S. Patents 5,892,628 and 5,872,261.

Specifications

Description	EZ-Link Sulfo-NHS-Biotinylation Kit
Quantity	10 reactions
Labeling Scale	1 to 10 mg
Solubility	Water
Labeling Target	Antibodies, Proteins

Product Type	Sulfo-NHS-Biotinylation Kit
Content And Storage	Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody • EZ-Link Sulfo-NHS-Biotin, 25 mg • PBS Pack (makes 500mL), 1 pack • Zeba Spin Desalting Columns (7K MWCO), 5 mL, 10 columns • HABA Solution, 1 mL • Avidin, 10 mg Store avidin and vial of biotin reagent (with desiccant) at -20°C. Store remaining kit components at 4°C.
Product Line	EZ-Link™
Label or Dye	Biotin

Product Suggestions Safety and Handling

Safety and Handling

Product Identifier

EZ-Link Sulfo-NHS-Biotinylation Kit

Signal Word

Warning

Hazard Category

Acute toxicity Category 4
Serious eye damage/eye irritation Category 2
Skin corrosion/irritation Category 2
Specific target organ toxicity Category 3

Hazard Statement

H302-Harmful if swallowed.
H312-Harmful in contact with skin.
H315-Causes skin irritation.
H319-Causes serious eye irritation.
H332-Harmful if inhaled.
H335-May cause respiratory irritation.

Precautionary Statement

P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
P264-Wash hands thoroughly after handling.
P270-Do not eat, drink or smoke when using this product.
P271-Use only outdoors or in a well-ventilated area.
P280-Wear protective gloves/protective clothing/eye protection/face protection.
P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
P302+P352-IF ON SKIN: Wash with plenty of water/soap.
P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P311-Call a POISON CENTER/doctor/ .
P312-Call a POISON CENTER/doctor/if you feel unwell.
P330-Rinse mouth.
P332+P313-If skin irritation occurs: Get medical advice/attention.
P337+P313-If eye irritation persists: Get medical advice/attention.
P362+P364-Take off contaminated clothing and wash it before reuse.
P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

MIXTURE LIST-Contains : Sulfo-NHS-Biotin, Sodium chloride, Sodium phosphate dibasic

Documents

Product Information

Manuals

User Guide: EZ-Link Sulfo-NHS-Biotinylation Kit Version: B File size: 356.2 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot NumberZJ397934A	Certificate TypeCertificate of Analysis	Date05/11/2024	Catalog Number21425
Lot NumberZJ397934	Certificate TypeCertificate of Analysis	Date05/11/2024	Catalog Number21425
Lot NumberZG394820	Certificate TypeCertificate of Analysis	Date07/08/2024	Catalog Number21425
Lot NumberZD386812	Certificate TypeCertificate of Analysis	Date01/05/2024	Catalog Number21425
Lot NumberZB384786	Certificate TypeCertificate of Analysis	Date02/03/2024	Catalog Number21425

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Product Title

Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotinylation Kit > Sulfo-NHS-Biotin spacer arm length 13.5Å

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