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Thermo Scientific™ TAE Buffer (Tris-acetate-EDTA) (50X)
Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA).
Brand: Thermo Scientific™ B49
513.22 DKK valid until 2024-12-31
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Description
Use the Thermo Scientific 50X TAE Buffer (Tris-acetate-EDTA) for electrophoresis of nucleic acids in agarose and polyacrylamide gels. You can use this buffer for both genomic and large supercoiled DNA, and you can also use this as both a running and a gel preparation buffer.
Usage Recommendations
- Use fresh 1X TAE both for the gel and for the electrophoresis run
- To prepare 1X TAE buffer add 20ml of 50X TAE buffer to 980ml of deionized water and mix well
Quality Control
- The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests
1X Composition
- 40mM Tris
- 20mM acetic acid
- 1mM EDTA
- pHof 50X TAE: 8.4
Recommended for:
Electrophoresis of nucleic acids in agarose and polyacrylamide gels; Used both as a running buffer and as a gel preparation buffer; Filtered through a 0.22μm membrane; Recommended for resolution of RNA and DNA fragments l.
Note:
TAE buffer has a relatively low buffering capacity, therefore periodic replacement of the buffer during prolonged electrophoresis is recommended.
Specifications
| 50X | |
| 1 L | |
| TAE Buffer |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- TAE Buffer (Tris-acetate-EDTA) (50X)
Signal Word
- Warning
Hazard Category
- Specific target organ toxicity after repeated exposure Category 2
Hazard Statement
- H373-May cause damage to organs.
Precautionary Statement
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P314-Get medical advice/attention if you feel unwell.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : acetic acid
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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