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Thermo Scientific™ HinfI, HC (50 U/μL)

Thermo Scientific™ Fermentas HinfI is available as a FastDigest enzyme which is used for rapid DNA digestion.

Brand:  Thermo Scientific™ ER0803

Product Code. 10579120

  • 1572.00 DKK / 10000 units

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Description

Description

Thermo Scientific™ HinfI is available as a FastDigest enzyme which is used for rapid DNA digestion. More than 95% of the digested DNA fragments can be ligated and recut at a 5'-termini concentration of 1.3μM after a 50-fold overdigestion (3U/μg DNA x 17 hours) with HinfI.

5'...G▵A N T C...3'

3'...C T N A▵G...5' Conditions for 100% Activity:

  • 1X Buffer R: 10mM Tris-HCl (pH8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • HinfI is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: may overlap —cleavage impaired
  • EcoKI: never overlaps —no effect
  • EcoBI: may overlap —blocked

Compatible Ends:

  • G▴AWTC — PfeI
TRUSTED_SUSTAINABILITY
Specifications

Specifications

50 U/μL
Hinf I
Yes
No
Restriction Enzyme
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
10X Buffer R
37°C
10,000 units
Traditional Cloning
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Product Information

Manuals

User Guide: HinfI, HC, 50 U/uL, 10000U Version: Jan. 2015 File size: 65.4 kB
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