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Thermo Scientific™ KpnI, HC (50 U/μL)

The KpnI restriction enzyme recognizes GGTAC^C sites and cuts best at 37°C in its own unique buffer (Isoschizomers: Asp718I).

Brand: Thermo Scientific™ ER0521

540.00 DKK valid until 2025-04-18
Use promo code "24107" to get your promotional price.

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Product Code. 10328759

772.00 DKK / 4000 units

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Description

Lambda DNA digested with KpnI, 0.7% agarose, 2 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'..G G T A C▵C...3'

3'..C▵C A T G G...5'

Conditions for 100% Activity:

1X Buffer KpnI: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl₂, 0.02% Triton X-100 and 0.1mg/mL BSA
Incubate at 37°C

Storage Buffer:

KpnI is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

Dam: never overlaps —no effect
Dcm: may overlap —no effect
CpG: may overlap —no effect
EcoKI: never overlaps —no effect
EcoBI: never overlaps —no effect

Digestion of Agarose-embedded DNA:

Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Note:

High glycerol (>5%) concentrations, pH>8.0 or a large excess of enzyme may result in star activity.

Specifications

Concentration	10 U/μL
Enzyme	Kpn I
Sensitive to Heat Inactivation	Yes
Type IIS RE	No
Product Type	Restriction Enzyme

Methylation Sensitivity	Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer	Unique Buffer (10X Buffer KpnI)
Optimal Reaction Temperature	37°C
Quantity	4,000 units
Research Category	Traditional Cloning

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Documents

Product Information

Manuals

Digestion of agarose-embedded DNA Version: Rev B (18 March 2016) File size: 96.3 kB

User Guide: KpnI, 10 U/uL, 5x4000U Version: Jan. 2015 File size: 66 kB

User Guide: KpnI, 10 U/uL, 4000U Version: Jan. 2015 File size: 66 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2834025	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberER0521
Lot Number2860024	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog NumberER0521
Lot Number2867393	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog NumberER0521
Lot Number2902483	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog NumberER0521
Lot Number2902551	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog NumberER0521

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