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Thermo Scientific™ T3 RNA Polymerase (20 U/μL)

Thermo Scientific™ Fermentas T3 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for its double-stranded promoter.

Brand:  Thermo Scientific™ EP0101

213.34 DKK valid until 2025-03-21
Use promo code "24111" to get your promotional price.

Product Code. 10262890

  • 320.00 DKK / 2000 units

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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24111” to get your promotional price

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Description

Description

Thermo Scientific™ Fermentas Bacteriophage T3 RNA polymerase is DNA-dependent RNA polymerase with strict specificity for their respective double-stranded promoters.

It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from its promoter.
  • Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)

Recommended for:

Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.

Note:

Consensus promoter sequences: AATTAACCCTCACTAAA G GGAGA–T3 TAATACGACTCACTATA G GGAGA–T7 ATTTAGGTGACACTATA G AAGNG–SP6 The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively (9).

TRUSTED_SUSTAINABILITY
Specifications

Specifications

T3 RNA Polymerase
2,000 units
20U/µL
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Product Information

Manuals

User Guide: Conventional in vitro Transcription Version: Jan. 2015 File size: 85.2 kB
User Guide: T3 RNA Polymerase Version: MAN0012019 Rev. B.0 (16Sep2016) File size: 234.6 kB
User Guide: DNA Template Preparation for in vitro Transcription Version: Jan. 2015 File size: 101.7 kB
User Guide: Synthesis of Radiolabeled RNA Probes of High Specific Activity Version: Jan. 2015 File size: 84.4 kB
Certificates

Certificates

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5 results found
Lot Number Certificate Type Date Catalog Number
Lot Number2828077 Certificate TypeCertificate of Analysis Date31/01/2025 Catalog NumberEP0101
Lot Number2849505 Certificate TypeCertificate of Analysis Date28/01/2025 Catalog NumberEP0101
Lot Number2877197 Certificate TypeCertificate of Analysis Date28/01/2025 Catalog NumberEP0101
Lot Number2892146 Certificate TypeCertificate of Analysis Date28/01/2025 Catalog NumberEP0101
Lot Number2892131 Certificate TypeCertificate of Analysis Date28/01/2025 Catalog NumberEP0101
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Thermo Scientific™ T3 RNA Polymerase (20 U/μL) > 20u/uL, 2000U

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