missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Invitrogen™ Galacto-Star™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells
The Galacto-Star™ One-Step β-Galactosidase Reporter Gene Assay System chemiluminescent reporter gene assay system enables rapid, sensitive detection of β-galactosidase in mammalian cell lysates. SensitiveThe chemiluminescent assay exhibits over three o
Brand: Invitrogen™ T1012
Includes: Lysis solution, substrate, enhancer, and required buffer
Description
- Glow assay kinetics provides a “window” during which measurements may be performed, facilitating HTS applications where assay automation is used
- Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems
- Wide dynamic range of β-galactosidase assay lets the user measure enzyme level accurately from femtogram to nanogram range
- Assay sensitivity is 100- to 1,000-fold better than either the isotopic/non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric/fluorescent assays for β-galactosidase, providing greater sensitivity than competing assays
- Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions
- Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes
- Assay can be completed in about one hour, providing fast assay turnaround
Sensitive
The chemiluminescent assay exhibits over three orders of magnitude greater sensitivity than colorimetric β-galactosidase assays and can be completed in a fraction of the time required for assays for chloramphenicol acetyltransferase (CAT) and ELISA-based reporter assays.
Versatile
The Galacto-Star system includes Galacton-Star chemiluminescent substrate for β-galactosidase and is suited for measurement in both luminometers and scintillation counters. Automatic injection is not required.
Long-Lasting Signal
Light emission from the Galacto-Star assay reaches maximum in 60 to 90 minutes and remains constant for nearly one hour. Cell lysate is mixed with reaction buffer containing Galacton-Star substrate and Sapphire-II™ luminescence enhancer. After a 60-90 minute incubation at room temperature, the signal is measured in a luminometer or scintillation counter.
Specifications
- Assay Type: β-Gal (lacZ) Assay
- Compatible Cells: Mammalian Cells
- Detection Method: Chemiluminescent
- For Use With Equipment: Luminometer (Microplate)
- Form: Liquid
- Format: 96-well plate
- High Throughput Compatibility: High Throughput-Compatible
- Readout: End Point
- Sample Type (Specific): Cell Cultures
- Substrate: Galacton-Star
- Substrate Properties: Chemical Substrate
- Substrate Type: Beta-Gal Substrate
- Target Enzyme: Beta-Galactosidase
Specifications
| β-Galactosidase Reporter Gene Assay System | |
| Luminometer (Microplate) | |
| This kit contains the lysis solution, substrate, enhancer, and required buffer. Store the product at 4°C. | |
| High-throughput Compatible | |
| End Point | |
| Galacton-Star™ | |
| Chemical Substrate | |
| Galacto-Star™, NovaBright™ | |
| 600 Assays |
| Chemiluminescence | |
| β-Gal (lacZ) Assay | |
| Mammalian Cells | |
| Unconjugated | |
| Cell Cultures | |
| Beta-Gal Substrate | |
| Beta-Galactosidase | |
| Enzyme Assay | |
| Liquid |
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
For Research Use Only. Not for use in diagnostic procedures.
Spot an opportunity for improvement?Share a Content Correction