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Invitrogen™ Galacto-Star™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells
The Galacto-Star™ One-Step β-Galactosidase Reporter Gene Assay System chemiluminescent reporter gene assay system enables rapid, sensitive detection of β-galactosidase in mammalian cell lysates. SensitiveThe chemiluminescent assay exhibits over three o
Brand: Invitrogen™ T1014
Includes: Lysis solution, substrate, enhancer, and required buffer
Description
- Glow assay kinetics provides a “window” during which measurements may be performed, facilitating HTS applications where assay automation is used
- Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems
- Wide dynamic range of β-galactosidase assay lets the user measure enzyme level accurately from femtogram to nanogram range
- Assay sensitivity is 100- to 1,000-fold better than either the isotopic/non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric/fluorescent assays for β-galactosidase, providing greater sensitivity than competing assays
- Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions
- Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes
- Assay can be completed in about one hour, providing fast assay turnaround
Sensitive
The chemiluminescent assay exhibits over three orders of magnitude greater sensitivity than colorimetric β-galactosidase assays and can be completed in a fraction of the time required for assays for chloramphenicol acetyltransferase (CAT) and ELISA-based reporter assays.
Versatile
The Galacto-Star system includes Galacton-Star chemiluminescent substrate for β-galactosidase and is suited for measurement in both luminometers and scintillation counters. Automatic injection is not required.
Long-Lasting Signal
Light emission from the Galacto-Star assay reaches maximum in 60 to 90 minutes and remains constant for nearly one hour. Cell lysate is mixed with reaction buffer containing Galacton-Star substrate and Sapphire-II™ luminescence enhancer. After a 60-90 minute incubation at room temperature, the signal is measured in a luminometer or scintillation counter.
Specifications
- Assay Type: β-Gal (lacZ) Assay
- Compatible Cells: Mammalian Cells
- Detection Method: Chemiluminescent
- For Use With Equipment: Luminometer (Microplate)
- Form: Liquid
- Format: 96-well plate
- High Throughput Compatibility: High Throughput-Compatible
- Readout: End Point
- Sample Type (Specific): Cell Cultures
- Substrate: Galacton-Star
- Substrate Properties: Chemical Substrate
- Substrate Type: Beta-Gal Substrate
- Target Enzyme: Beta-Galactosidase
Specifications
| β-Galactosidase Reporter Gene Assay System | |
| Luminometer (Microplate) | |
| This kit contains the lysis solution, substrate, enhancer, and required buffer. Store the product at 4°C. | |
| High-throughput Compatible | |
| End Point | |
| Galacton-Star™ | |
| Chemical Substrate | |
| Galacto-Star™, NovaBright™ | |
| 1800 Assays |
| Chemiluminescence | |
| β-Gal (lacZ) Assay | |
| Mammalian Cells | |
| Unconjugated | |
| Cell Cultures | |
| Beta-Gal Substrate | |
| Beta-Galactosidase | |
| Enzyme Assay | |
| Liquid |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Danger
Hazard Category
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H225-Highly flammable liquid and vapour.
- H319-Causes serious eye irritation.
- H335-May cause respiratory irritation.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P271-Use only outdoors or in a well-ventilated area.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P370+P378-In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
- P403+P233-Store in a well-ventilated place. Keep container tightly closed.
- P403+P235-Store in a well-ventilated place. Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : isopropanol
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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