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Invitrogen™ GeneArt™ CRISPR Nuclease mRNA
Invitrogen™ GeneArt™ CRISPR Nuclease mRNA is formulated using a new manufacturing process to achieve superior quality and improved product performance.
Brand: Invitrogen™ A29378
This item is not returnable.
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Description
- This wild-type Cas9 mRNA has two nuclear localization signals (NLSs).
- Cas9 constructs with two NLSs have been shown to be more efficient at targeting the Cas9 protein to the nucleus (1).
- The mRNA format is ready to transfect and circumvents the need for time-consuming cloning steps required when using CRISPR vector systems.
- Cas9 mRNA is co-transfected with a target-specific guide RNA (gRNA) to direct the Cas9 protein to the intended genome locus to create a double-stranded break.
- The complete RNA format is a smaller payload than plasmid-based Cas9 systems for improved delivery into the cell and better genome editing efficiency.
- Additionally, the Cas9 mRNA can be used in multiplex approaches with more than one gRNA.
- Use this approach to determine which gRNA sequence works best for a particular target or to edit multiple genomic loci with one transfection.
Specifications
| 1 vial CRISPR Nuclease mRNA. Store in ultra-cold freezer -68 to -85°C | |
| 15 μg |