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Thermo Scientific™ GeneBLAzer™ C5AR1-Gα15 CHO-K1 DA Assay Kit
The GeneBLAzer™ C5AR1-Gα15 CHO-K1 DA (Division Arrested) cells contain the human Complement Component 5a Receptor 1 (C5AR1) stably integrated into the GeneBLAzer™ Gα15-NFAT-bla CHO-K1 cell line.
Brand: Thermo Scientific™ K1544
This item is not returnable.
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Description
The GeneBLAzer™ C5AR1-Gα15 CHO-K1 DA (Division Arrested) cells contain the human Complement Component 5a Receptor 1 (C5AR1) (Accession No. NM_000710) stably integrated into the GeneBLAzer™ Gα15-NFAT-bla CHO-K1 cell line. GeneBLAzer™ Gα15-NFAT-bla CHO-K1 (Cat. No. K1213) contains a beta-lactamase (bla) reporter gene under control of a NFAT response element and a promiscuous G Protein, Gα15, stably integrated into CHO-K1 cells.
The C5AR1-Gα15 CHO-K1 DA (Division Arrested) cells cells are functionally validated for Z'-factor and EC50 concentrations using C5a. In addition, C5AR1-Gα15-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
Specifications
| NM_001736.2 | |
| Division-Arrested Cells | |
| GeneBLAzer™ C5AR1-Gα±15 CHO-K1 DA Assay Kit, 1 Plate, ∽4,800,000 cells/vial Quantity of Cells, CHO-K1 Cell Line, Fluorescent Detection Method, GPCRs Druggable Target, BLA (Beta-Lactamase) Reporter, GeneBLAzer™ System, Liquid Nitrogen Storage | |
| Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays | |
| Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol). Includes: GeneBLAzer™ C5AR1-G?15 CHO-K1DA cells, LiveBLAzer™-FRET B/G Loading Kit, 70μg, Solution D, GeneBLAzer™ C5AR1-G?15 CHO-K1Assay Protocol, LiveBLAzer™-FRET B/G Loading Protocol, Certificate of Analysis | |
| GeneBLAzer™ | |
| BLA (Beta-Lactamase) | |
| C5AR1 | |
| CHO-K1 | |
| GPCR |
| Cell-based beta lactamase reporter gene | |
| Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol). Includes: • GeneBLAzer™ C5AR1-Gα15 CHO-K1DA cells • LiveBLAzer™-FRET B/G Loading Kit, 70 μg • Solution D • GeneBLAzer™ C5AR1-Gα15 CHO-K1 Assay Protocol • LiveBLAzer™-FRET B/G Loading Protocol • Certificate of Analysis The GeneBLAzer™ C5AR1-Gα15 CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
|
| Fluorescence | |
| C5aR1 | |
| Single Cell Clone Origin | |
| 1 Plate | |
| CHO-K1 | |
| Division-Arrested Cells Assay Kit | |
| GeneBLAzer™ |
For Research Use Only. Not for use in diagnostic procedures.