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Thermo Scientific™ GeneBLAzer™ CALCR -CRE-bla FreeStyle™ 293F Cells
The GeneBLAzer™ CALCR-CRE-bla Freestyle293 cells contain the CALCR receptor isoform (CTR2/CT(a)/CTRI1-), Accession No. NM_001742.2, stably integrated into the CellSensor™ CRE-bla Freestyle293 cell line.
Brand: Thermo Scientific™
This item is not returnable.
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Description
The GeneBLAzer™ CALCR-CRE-bla Freestyle293 cells contain the CALCR receptor isoform (CTR2/CT(a)/CTRI1-), Accession No. NM_001742.2, stably integrated into the CellSensor™ CRE-bla Freestyle293 cell line. The CellSensor™ CRE-bla Freestyle293 cells contain a beta-lactamase (bla) reporter gene under control of the cAMP (CRE) response element.
CALCR-CRE-bla Freestyle293 cells have been tested for variable assay conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time and functionally validated for Z' and EC50 concentrations of calcitonin. Additional testing data using alternate stimuli are also provided. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.
Specifications
| NM_001742.2 | |
| Dividing Cells | |
| GeneBLAzer™ CALCR -CRE-bla FreeStyle™ 293F Cells, 2 x 106 Cells, ∽2,000,000 cells/vial at 2,000,000 cells/mL Quantity of Cells, FreeStyle™ 293-F Cell Line, Fluorescent Detection Method, GPCRs Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage | |
| Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays | |
| GeneBLAzer™ CALCR -CRE-bla FreeStyle™ 293F Cells (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: GeneBLAzer™ CALCR-CRE-bla Freestyle™ 293F cells, GeneBLAzer™ CALCR-CRE-bla Freestyle™ 293F Assay Protocol, Certificate of Analysis | |
| GeneBLAzer™ | |
| BLA (Beta-Lactamase) | |
| CALCR2 | |
| FreeStyle 293-F Cells | |
| GPCR |
| Cell-based beta lactamase reporter gene | |
| LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol. Includes: • GeneBLAzer™ CALCR-CRE-bla Freestyle™ 293F cells • GeneBLAzer™ CALCR-CRE-bla Freestyle™ 293F Assay Protocol • Certificate of Analysis The GeneBLAzer™ CALCR-CRE-bla Freestyle™ 293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
|
| Fluorescence | |
| CALCR2 | |
| Single Cell Clone Origin | |
| 2 x 106 Cells | |
| FreeStyle™ 293-F | |
| Cell Line | |
| GeneBLAzer™ |
For Research Use Only. Not for use in diagnostic procedures.