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Thermo Scientific™ GeneBLAzer™ NTSR1 CHO-K1 DA Assay Kit
The GeneBLAzer™ NTSR1 CHO-K1 DA cells contain the human Neurotensin Receptor 1 (NTSR1) stably integrated into the GeneBLAzer™ Gqo5-NFAT-bla CHO-K1 cell line.
Brand: Thermo Scientific™ K1773
This item is not returnable.
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Description
The GeneBLAzer™ NTSR1 CHO-K1 DA cells contain the human Neurotensin Receptor 1 (NTSR1) stably integrated into the GeneBLAzer™ Gqo5-NFAT-bla CHO-K1 cell line. CellSensor™ NFAT-bla CHO-K1 cells (Cat. No. K1078) contain a beta-lactamase reporter gene under control of the NFAT response element.
The GeneBLAzer™ NTSR1 CHO-K1 DA cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, GeneBLAzer™ NTSR1 CHO-K1 DA cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
Specifications
| NM_002531 | |
| Division-Arrested Cells | |
| Fluorescence | |
| NTSR1 | |
| GeneBLAzer™ | |
| 1 Kit | |
| BLA (Beta-Lactamase) | |
| Division-Arrested Cells Assay Kit | |
| GeneBLAzer™ |
| Cell-based beta lactamase reporter gene | |
| LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol. Includes: • GeneBLAzer™ NTSR1 CHO-K1 DA cells • LiveBLAzer™-FRET B/G Loading Kit, 70 μg • Solution D • GeneBLAzer™ NTSR1 CHO-K1 DA Assay Protocol • LiveBLAzer™-FRET B/G Loading Protocol • Certificate of Analysis The GeneBLAzer™ NTSR1 CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
|
| GPCRs | |
| Pool of Stable Cells | |
| GPCR (G-Protein Coupled Receptors) | |
| End Point | |
| NTSR1 | |
| U2OS | |
| GPCR |
For Research Use Only. Not for use in diagnostic procedures.