missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Thermo Scientific™ GeneBLAzer™ VDR DA Assay Kit
GeneBLAzer™ VDR DA (Division Arrested) cells and VDR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Vitamin D receptor (VDR) fused to the DNAbinding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.
Brand: Thermo Scientific™ K1417
This item is not returnable.
View return policy
Description
GeneBLAzer™ VDR DA (Division Arrested) cells and VDR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Vitamin D receptor (VDR) fused to the DNAbinding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™ UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-VDR (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both VDR DA cells and VDR-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of Calcitriol (1α, 25-dihydroxyvitamin D3). In addition, VDR-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time.
Specifications
| Cell-based beta lactamase reporter gene | |
| Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate: • VDR HEK 293T DA cells (K1417A) • LiveBLAzer™-FRET B/G Loading Kit, 70 μg Shipping: Dry Ice Storage: -80°C (liquid nitrogen) |
|
| Nuclear Receptors | |
| VDR | |
| TO901317 | |
| 1 Plate | |
| VDR, NR1I1 | |
| 293T (HEK) | |
| Nuclear Receptors |
| Division-Arrested Cells | |
| Fluorescence | |
| 7421 | |
| VDR HEK 293T DA cells (K1417A) LiveBLAzer™-FRET B/G Loading Kit, 70 μg | |
| GeneBLAzer™ | |
| BLA (Beta-Lactamase) | |
| Division-Arrested Cells Assay Kit | |
| GeneBLAzer™ |
For Research Use Only. Not for use in diagnostic procedures.