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Gibco™ Advanced DMEM/F-12 Product Code.: 11550446
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Gibco™ Advanced DMEM/F-12
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Product Code. 11550446
10 x 500 mL, Pack of 10
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Quantity:
500 mL
10 x 500 mL
Unit Size:
500mL
Pack of 10
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Product Code. 11550446

Brand: Gibco™ 12634028

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This item is not returnable. View return policy
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24112” to get your promotional price

Requires

Supplementation

Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM/F-12, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM/F-12, with no adaptation, include MRC-5, SP2, Vero, WI-38 and Jurkat.

This Advanced DMEM/F-12 is manufactured as follows:

With: Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red

Without: L-glutamine, HEPES

The complete formulation is available.

Using Advanced DMEM/F-12

Advanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ II lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM/F-12 requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced Advanced DMEM/F-12 uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Cell Line MRC-5, SP2, Vero, WI-38 and Jurkat
Classification Animal Origin
Concentration 1 X
Form Liquid
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Serum Level Reduced Serum
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives High Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
Without Additives No Glutamine, No HEPES
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco™
Quantity 10 x 500 mL
Shipping Condition Room Temperature
Content And Storage Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
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Product Identifier
  • Methyl benzoate
Signal Word
  • Warning
Hazard Category
  • Acute toxicity Category 4
Hazard Statement
  • H302-Harmful if swallowed.
Precautionary Statement
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
Supplemental information
  • MIXTURE LIST-Contains : Methyl benzoate

For Research Use Only. Not for use in diagnostic procedures.

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