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Gibco™ DMEM, low glucose, pyruvate
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.
Brand: Gibco™ 11885084
Description
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
With: Low Glucose, L-Glutamine, Sodium Pyruvate, Phenol Red
Without: HEPES
The complete formulation is available.
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Product use
For human ex vivo tissue and cell culture processing applications. CAUTION: When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco™ DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).
Order Info
Shipping Condition: Room Temperature
Specifications
| Storage conditions: 2°C to 8°C (protect from light) Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
|
| Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells | |
| 1 X | |
| DMEM (Dulbecco's Modified Eagle Medium) | |
| Sterile-filtered | |
| Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate | |
| cGMP-compliant under the ISO 13485 standard | |
| 500 mL |
| HeLa, 293, Cos-7, and PC-12 | |
| Animal Origin-free | |
| Liquid | |
| Standard Serum Supplementation | |
| Sterile-filtered | |
| No HEPES | |
| Gibco™ | |
| Room Temperature |
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Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
5 results found
| Lot Number | Certificate Type | Date | Catalog Number |
|---|---|---|---|
| 2508906 | Certificate of Analysis | 29/01/2025 | 11885084 |
| 2537084 | Certificate of Analysis | 29/01/2025 | 11885084 |
| 2715711 | Certificate of Analysis | 29/01/2025 | 11885084 |
| 2715705 | Certificate of Analysis | 29/01/2025 | 11885084 |
| 2715713 | Certificate of Analysis | 29/01/2025 | 11885084 |
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Product Title
Gibco™ DMEM, low glucose, pyruvate > Quantity: 500 mL
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
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