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Description
Requires
Supplementation
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
With: L-glutamine, Phenol Red
Without: Glucose, Sodium Pyruvate, HEPES
The complete formulation is available.
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature
- Environmental benefits include:
- Recycled Content
- Renewable Materials
- Recyclable
- Source Reduction

Specifications
Specifications
Cell Line | HeLa, 293, Cos-7, and PC-12 |
Cell Type | Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells |
Classification | Animal Origin-free |
Concentration | 1 X |
Form | Liquid |
Product Type | DMEM (Dulbecco's Modified Eagle Medium) |
Serum Level | Standard Serum Supplementation |
Sterility | Sterile-filtered |
With Additives | Glutamine, Phenol Red |
Without Additives | No Glucose, No HEPES, No Sodium Pyruvate |
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Safety and Handling


- o-Fluorotoluene
- Danger
- Flammable liquid Category 2
- Specific target organ toxicity Category 3
- H225-Highly flammable liquid and vapour.
- H335-May cause respiratory irritation.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P240-Ground/bond container and receiving equipment.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- MIXTURE LIST-Contains : o-Fluorotoluene
For Research Use Only. Not for use in diagnostic procedures.
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