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  7. Gibco™ Ham's F-12 Nutrient Mix, GlutaMAX™ Supplement
  8. 15344967

Gibco™ Ham's F-12 Nutrient Mix, GlutaMAX™ Supplement
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Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells.

Brand:  Gibco™ 31765035

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Product Code. 15344967

  • 375.00 DKK / 500mL

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Requires : Supplementation
This item is not returnable. View return policy

Description

Description

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of Gibco™ F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Ham's F-12 is modified as follows:

With: GlutaMAX™, Phenol Red

The complete formulation is available.

Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations. Gibco™ F-12 with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Gibco™ F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

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Specifications

Specifications

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Primary Rat Astrocytes
1 X
Ham's F-12 Nutrient Mixture
Sterile-filtered
No HEPES
Gibco™, GlutaMAX™
Room Temperature
CHO, COS-7, and rat prostate epithelial cells
Animal Origin-free
Liquid
Sterile-filtered
GlutaMAX, Phenol Red, Sodium Pyruvate
cGMP-compliant under the ISO 13485 standard
500 mL
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For Research Use Only. Not for use in diagnostic procedures. Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.