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Description
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM is modified as follows:
With: Earle's salts, Phenol Red
Without: L-glutamine, HEPES, Calcium
The complete formulation is available.
MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification. This MEM formulation is made without calcium for use with suspension cultures. This MEM formulation contains Earle's salts for use in a CO2 incubator. This product is made with Earle's salts.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.
MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature
Specifications
| Storage conditions: 2-8°C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
|
| Primary Rat Astrocytes | |
| 1 X | |
| MEM (Minimum Essential Medium) | |
| Sterile-filtered | |
| No Glutamine, No HEPES, No Sodium Pyruvate, No Calcium | |
| Gibco™ | |
| Room Temperature |
| HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts | |
| Animal Origin-free | |
| Liquid | |
| Standard Serum Supplementation | |
| Low Glucose, Phenol Red, Earle's Balanced Salt Solution (EBSS) | |
| cGMP-compliant under the ISO 13485 standard | |
| 500 mL |
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Product Title
Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law. For in vitro diagnostic use.
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