missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Hela (human cervical epithelioid carcinoma) Whole cell lysate, Denatured; Abnova
Click to view available options
Quantity:
200 μg
Unit Size:
200µg
Description
- Tissue: Cervix
- Host: Human
- Lysis buffer: Modified RIPA lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
- Storage buffer: In ready-to-use 1X Sample Buffer (50mM Tris-HCl, 2% SDS, 10% glycerol, 300mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Western Blotting
Specifications
Specifications
| For Use With (Application) | Western Blot |
| Tissue | Cervix |
| Description | Whole cell lysate (denatured) |
| Lysis Buffer | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. |
| Preparation Method | Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was mixed in 5X Sample Buffer to become final 1X Sample Buffer as Storage Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly. |
| Quality Control Testing | 12.5% SDS-PAGE Stained with Coomassie Blue. |
| Quantity | 200 μg |
| Storage Buffer | In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue). |
| Host Species | Human |
| Content And Storage | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
Hela (human cervical epithelioid carcinoma) Whole cell lysate, Denatured; Abnova >
Spot an opportunity for improvement?Share a Content Correction