HisPur Cobalt Resins, Columns and Kits use a tetradentate chelating resin charged with divalent cobalt (Co2+)to enable specific, fast and gentle purification of histidine-tagged fusion proteins from a variety of sample types. HisPur Cobalt Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high throughput needs. With these options, high-quality His-tag protein purification is possible at nearly any scale. HisPur Cobalt Resin is superior to traditional nickel-based immobilized metal affinity chromatography (IMAC) systems. Compared to Ni-IDA and tetradentate Ni2+chelate resins, HisPur Resin binds histidine-tagged proteins with higher specificity (less off-target binding) and releases them with gentler conditions (lower concentrations of imidazole). Use HisPur Columns and Cartridges to obtain high-purity His-tagged proteins with no metal contamination.
Highlights:
High purity – obtain more than 10 milligrams of pure His-tagged protein per milliliter of resin without optimizing imidazole washing conditions
Low metal leaching – no metal contamination in eluted histidine-tagged protein sample
Versatility – purify proteins under native or denaturing conditions; compatible with cell lysis reagents and a variety of buffer additives
Flexibility – available as bulk resin or predispensed columns, chromatography columns and 96-well filter plates
Cost effective – resin can be reused several times or discarded after single use
Superior – performs better than other commercially available IMAC Resins
Purification Kits – complete kits in all three column sizes
Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing
96-well Spin Plates – pre-dispensed 96-well filter plates, compatible with centrifugation and vacuum manifold systems for manual or automated purification. The plates enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations
Bulk and Custom – let us know your needs for bulk HisPur Cobalt Resin
Includes:
Kits contain HisPur Cobalt Columns, equilibration/wash buffer and elution buffer
