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Cytiva HisTrap™ excel Prepacked Columns

For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.

560.00 DKK - 7215.00 DKK

Specifications

Description HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
Material Polypropylene
pH Range 2 to 12
Support Type 6% Highly Cross-linked Agarose
Content And Storage 4°C to 30°C
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Products 3
Product Code Brand Diameter (Metric) Inner Quantity Flow Rate Price Quantity & Availability  
Product Code Brand Diameter (Metric) Inner Quantity Flow Rate Price Quantity & Availability  
12632966
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Cytiva
17-3712-05
7 mm 5 x 1 mL 1 to 4 mL/min.
2145.00 DKK
5mL
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12683466
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Cytiva
17-3712-06
16 mm 5 x 5 mL 5 to 20 mL/min.
7215.00 DKK
Pack of 5
In Stock
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15187946
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Cytiva
29048586
7 mm 1 mL 1 to 4 mL/min.
560.00 DKK
1mL
In Stock
to see stock.
Add to basket
 
Description

Description

The design of the columns in combination with the specific properties of the medium enables fast and convenient purifications.

  • Ready-to use prepacked with Ni Sepharose excel
  • Special type of filter in top and bottom of the columns to load large volumes of cell-free, un clarified samples directly on the columns helps prevent degradation and loss of sensitive target proteins
  • Made of biocompatible polypropylene and cannot be opened or refilled
  • Delivered with stopper on inlet and snap-off end on outlet
  • Operated either with peristaltic pump or with chromatography systems such as KTA systems
  • Binding Capacity/Column: At least 10 mg histidine-tagged protein/ml sedimented medium
Media
  • Average particle size: 90μm
  • Matrix: Highly cross-linked agarose, 6%
  • pH Stability working range: 2 to 12
  • pH Stability cleaning range: 2 to 14
  • Flow velocity: 150 cm/h - 600 cm/h4
Column:
  • Column I.D.: 7 mm

HisTrap excel 1mL column

  • Bed Dimensions: 7 × 25mm
  • Flow rate: 1 to 4mL/min.
HisTrap excel 5mL column
  • Bed Dimensions: 16 × 25mm
  • Flow rate: 5 to 20mL/min.

Specifications

Specifications

HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
2 to 12
4°C to 30°C
Pre-packed Column
Polypropylene
6% Highly Cross-linked Agarose
5 bar (0.5 MPa, 70 psi)
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Safety and Handling

Safety and Handling

Signal Word
  • Warning

Hazard Category
  • Flammable liquid Category 3

Hazard Statement
  • H226-Flammable liquid and vapour.

Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501-Dispose of contents/container to .

Supplemental information
  • MIXTURE LIST-Contains : ethanol

SDS
Documents

Documents

Product Certifications