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Cytiva HisTrap™ FF Columns: 16 x 25mm
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Quantity:
5 x 1 mL
100 x 1 mL
Unit Size:
Pack of 100
Pack of 5
Description
- Prepacked with precharged Ni Sepharose™ 6 Fast Flow media
- Column material: polypropylene
- Bed dimensions: 16 × 25mm
- Flow velocity: <20mL/min.
- Protein binding capacity of ∽40mg/mL media with negligible nickel ion leakage
- Compatible with wide range of reducing agents, detergents, denaturants, and other additives
- Simple operation with syringe, perstaltic pump, or chromatographic system
- Columns may be connected in series to increase capacity
Medium
- Matrix: 6% cross-linked agarose
- Average particle size: 90μm
- pH stability working range: 3 to 12

Specifications
Specifications
Bed Volume | 5 |
Diameter (English) Inner | 0.6 in. |
Diameter (Metric) Inner | 16 mm |
Description | HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). |
Flow Rate | <20 mL/min. |
Material | Polypropylene |
pH Range | 3 to 12 |
Support Type | 6% Cross-linked Agarose |
Content And Storage | 4°C to 30°C, 20% Ethanol |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
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Safety and Handling

Signal Word
- Warning
- Flammable liquid Category 3
- H226-Flammable liquid and vapour.
- EUH208-Contains nickel. May produce an allergic reaction.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P235-Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- P405-Store locked up.
- MIXTURE LIST-Contains : ethanol, Nickel
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