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Proteintech™ Human PLA2G1B ELISA Kit

Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous human PLA2G1B

Brand:  Proteintech™ KE00098-96T


Product Code. 16881506

  • 2007.00 DKK / 96 tests

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Description

Description

  • Recognizes human PLA2G1B
  • PLA2G1B (Phospholipase A2 group IB) is a secreted member of phospholipase A2 (PLA2) class of enzymes
    • Produced by pancreatic acinar cells
    • PLA2G1B is a calcium-dependent enzyme which can catalyze hydrolysis of SN2 position of membrane glycerophospholipids to release arachidonic acid and lysophospholipids
      • Involved in cell contraction, cell proliferation and pathological response

Process:

  • Antibody specific for human PLA2G1B pre-coated onto microwells
  • Human PLA2G1B protein in samples captured by the coated antibody after incubation
  • After extensive washing, another antibody of biotinylated specific for human PLA2G1B is added to detect captured human PLA2G1B protein
  • For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent
  • Solution containing sulfuric acid used to stop color development and color intensity
    • Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm
Specifications

Specifications

NP_000919
0.08 ng/mL
Sandwich ELISA, Double Antibody
Plasma, Serum
Human
Human
<10%
Blue ice
4.5 hr.
0.094 to 96 ng/mL
ISO 13485
Coated ELISA Kit
DAB
5319
Solid
<10%
-20°C to +4°C
P04054
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