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Gibco™ Human VEGF-165 Recombinant Protein Product Code.: 10690920

Gibco™ Human VEGF-165 Recombinant Protein

Product Code. 10690920
10 μg, Each
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Quantity:
1 mg
10 μg
100 μg
Unit Size:
Each
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Product Code. 10690920

Brand: Gibco™ PHC9394

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This item is not returnable. View return policy

Recombinant Protein

Carrier-Free Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile, distilled water or appropriate buffered solution containing 0.1% BSA to regain full activity. Make any further dilutions of the reconstituted protein in buffered solution containing a carrier protein such as PBS with 0.1% BSA. Storage: Store the lyophilized protein at 2-8°C or -20°C for long term storage, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze-thaw cycles.

The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P15692
For Use With (Application) Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
Formulation protein with no preservative
Gene ID (Entrez) 7422
Molecular Weight (g/mol) 40 kDa
Name Human VEGF-165
Purification Method Sequential Chromatography
Quantity 10 μg
Endotoxin Concentration <0.1 ng/ μg
Gene Alias H-VEGF-165; MVCD1; M-VEGF-165; vascular endothelial growth factor 165 b; vascular endothelial growth factor A; vascular endothelial growth factor A121; vascular endothelial growth factor A165; vascular permeability factor; VEGF; VEGF164; VEGF165; Vegfa; VEGF-A; VPF
Common Name VEGF-165
Biological Activity ED50 < 6 ng/mL; determined by the dose-dependent proliferation of HUVECs.
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Sequence APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Content And Storage 4°C
Classification Carrier-Free
Bioassay Proliferation of HUVECs (Human Umbilical Vein Endothelial Cells)
Endotoxin Level <0.1 ng/μg
Shipping Condition Wet Ice
Specific Activity 1.0 x 106 - 1.7 x 105 units⁄mg
Expression System HEK293 cells
Protein Family Growth Factors & Receptors
Protein Form Recombinant, Ligand
Form Lyophilized
Protein Subtype VEGF (Vascular Endothelial Growth Factor)
Research Category Oncology, Angiogenesis, Stem Cell Research, Differentiation, Inflammation
Product Line Gibco™
Purity or Quality Grade ≥95% by SDS-PAGE and HPLC
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Certificates

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5 results found
Lot Number Certificate Type Date Product Code
Lot Number2907291 Certificate TypeCertificate of Analysis Date21/06/2024 Product CodePHC9394
Lot Number2839500 Certificate TypeCertificate of Analysis Date02/02/2024 Product CodePHC9394
Lot Number2614658 Certificate TypeCertificate of Analysis Date17/06/2023 Product CodePHC9394
Lot Number2509774 Certificate TypeCertificate of Analysis Date28/10/2022 Product CodePHC9394
Lot Number2456196 Certificate TypeCertificate of Analysis Date11/04/2022 Product CodePHC9394
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