missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Invitrogen™ AccuPrime™ Pfx DNA Polymerase
AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis.
Brand: Invitrogen™ 12344024
2808.75 DKK valid until 2024-12-31
Use promo code "21651" to get your promotional price.
Includes: AccuPrime Pfx DNA Polymerase (100μL, at 2.5U/μL) is provided with 10X AccuPrime reaction mix (1mL) that contains dNTPs and a separate vial (1mL) of 50mM MgSO4. Reagents are provided for 200 amplification reactions of 50μL each.
Description
AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3'→5' exonuclease) activity. Platinum anti-Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity (see figure). Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum Pfx alone.
Benefits of AccuPrime Pfx DNA Polymerase
- Greater than 26 times higher fidelity than Taq DNA polymerase
- Amplification of fragments up to 12 kb
- Minimized PCR optimization
Applications
- Amplification of DNA from complex genomic, viral, and plasmid templates
- RT-PCR
- High-fidelity PCR
- Hot-start PCR
Notes
- AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.
- The 10X AccuPrime Pfx reaction mix contains MgSO4, dNTPs, and thermostable proteins that enhance specific primer-template hybridization during each PCR cycle.
Order Info
Approved for shipment on Wet or Dry Ice
Specifications
| • AccuPrime Pfx DNA Polymerase (1 x 100 μL at 2.5 U/μL) • 10X AccuPrime Pfx Reaction Mix (1 x 1 mL) • 50 mM MgSO4 (1 x 1 mL) Store at -20°C. |
|
| Low | |
| Standard | |
| Wet or Dry Ice | |
| 26X | |
| 200 Reactions | |
| Separate Components | |
| 100 μL |
| Primer-probe | |
| AccuPrime Pfx DNA Polymerase | |
| 200 Reactions | |
| Hot-start PCR, High-fidelity PCR | |
| Built-In Hot Start | |
| Blunt | |
| 12 kb or less |
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
For Research Use Only. Not for use in diagnostic procedures.
Spot an opportunity for improvement?Share a Content Correction