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Invitrogen™ BaculoDirect™ C-Term Transfection Kit

Powerful and versatile eukaryotic system for high-level protein expression in insect cells

Brand:  Invitrogen™ 12562039

Product Code. 10665973

  • 8620.00 DKK / 1 set

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Includes: Five 0.3ÎĽg vials of BaculoDirect Linear DNA, 125ÎĽL of Cellfectin transfection reagent, and ganciclovir.
This item is not returnable. View return policy

Description

Description

  • Engineered to include attR sites for quick and efficient recombination with Gateway entry clone
  • Gene of interest is recombined from entry clone into BaculoDirect Linear DNA using simple, one-hour LR reaction
  • Resulting reaction mix contains recombinant baculovirus carrying gene of interest and is used directly to transfect insect cells
  • Eliminates need for transforming bacteria and isolating large bacmid or co-transfection of transfer vector and linear baculovirus DNA into insect cells togreatly reduce hands-on time
  • Purified baculovirus can be isolated in less than one week

  • Gateway Linear DNA: Designed for rapid cloning with Gateway entry clone and subsequent expression in Sf9 or Sf21 insect cells
  • Strong polyhedrin promoter for high-level expression
  • R sites for efficient recombination with attL-flanked Gateway entry vector
  • TK gene for negative selection using ganciclovir
  • N-terminal V5-His tag for detection with Invitrogens Anti-V5 Antibody and purification with nickel-chelating resin (BaculoDirect N-Term Linear DNA)
  • TEV protease cleavage site for removal of V5-His tag following purification (BaculoDirect N-Term Linear DNA)
  • C-terminal V5-His tag for detection with Invitrogens Anti-V5 Antibody and purification with nickel-chelating resin (BaculoDirect C-Term Linear DNA)

Baculovirus Production and Titering, Cloning, Gateway Cloning, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Polyhedrin
The BaculoDirect™ transfection kits include five 0.3 μg vials of BaculoDirect Linear DNA, 125 μL Cellfectin transfection reagent, and ganciclovir.

Store the BaculoDirect Linear DNA and Cellfectin at 4°C. All components are guaranteed stable for 6 months if stored properly.
Gateway
BaculoDirect Vectors
Transfection Kit
His Tag (6x), V5 Epitope Tag
5 transfections
BaculoDirect™, Gateway™
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Invitrogen™ BaculoDirect™ C-Term Transfection Kit > 5 transfections

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For Research Use Only. Not for use in diagnostic procedures.