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Invitrogen™ CIAP (Calf Intestinal Alkaline Phosphatase), 20 U/ÎĽL

Phosphomonoesterase that removes 3' and 5' phosphates from DNA and RNA

Brand:  Invitrogen™ 18009019

1006.46 DKK valid until 2025-06-30
Use promo code "24108" to get your promotional price.

Product Code. 10747864

  • 1274.00 DKK / 1ku

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Description

Description

  • Concentration: 20 units/ÎĽL
  • Source: Purified from calf intestinal mucosa
  • Performance and Quality Testing: Endodeoxyribonuclease, 3' exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency measured in a transformation assay
  • Unit Definition: One unit hydrolyzes 1ÎĽmol of 4-nitrophenyl phosphate in 1 min. at 37°C
  • Unit Reaction Conditions: 1M diethanolamine buffer, 10mM 4-nitrophenyl phosphate, 0.25mM MgCl2 (pH 9.8) in 900ÎĽL for 10 min. at 37°C

Dephosphorylation of 5'-phosphorylated termini of vector DNA to prevent self-ligation; Dephosphorylation of 5' termini of nucleic acids prior to forward reaction with kinase; Cloning; Restriction Enzyme Cloning

TRUSTED_SUSTAINABILITY
Specifications

Specifications

20 U/ÎĽL
Approved for shipment on Wet or Dry Ice
1000 U
Calf Intestinal Alkaline Phosphatase (20 units/μL) is supplied with a vial of 10X dephosphorylation buffer [500 mM Tris-HCl (pH 8.5), 1 mM EDTA], vial of dilution buffer [50% glycerol, 25 mM Tris-HCl (pH 7.6), 1 mM MgCl2 , and 0.1 mM ZnCl2 ]. Store Calf Intestinal Alkaline Phosphatase at -20°C.
CIP
Alkaline Phosphatase
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Invitrogen™ CIAP (Calf Intestinal Alkaline Phosphatase), 20 U/ÎĽL > 1000 units

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For Research Use Only. Not for use in diagnostic procedures.