Invitrogen™ One Shot™ OmniMAX™ 2 T1^R Chemically Competent E. coli

Description

The One Shot OmniMAX 2 T1^R E. coli strain is an improved chemically competent cell line that can achieve extremely high transformation efficiency of >5 x 10⁹ transformants/μg pUC19 DNA. It offers the highest transformation efficiency of any chemically competent cells in a One Shot format. OmniMAX 2 T1^R is a versatile strain, perfect to use in all cloning applications, including Gateway technology and TOPO PCR cloning.

OmniMAX 2 T1^R Chemically Competent E. coli cells carry the tonA (also known as fhuA) mutation, which confers resistance to T1 and T5 phage infection. The tonA genotype offers added security to valuable clones and libraries. This strain lacks the E. coli K12 restriction systems (mcrA Δ(mrr hsdRMS-mcrBC)), allowing efficient transformation of highly methylated DNA. The OmniMAX 2 T1^R strain has the lacZΔM15 genotype, allowing blue-white screening on plates containing either X-Gal or Bluo-Gal. It also carries the recA1 mutation, which helps reduce the rate of recombination while propagating plasmid DNA, and the endA1 mutation that increases plasmid DNA yield and quantity. The OmniMAX 2 T1^R strain contains an F´ episome that can support cloning plasmids with an f1-like origin of replication (phagemids). In addition, the F´ episome carries the lacIq repressor for inducible expression from trc, tac, and lac promoters using IPTG. Importantly, the ccdA gene present on the F´ episome is deleted, allowing negative selection of Gateway vectors as the strain is sensitive to the ccdB gene product.

One Shot OmniMAX 2 T1^R Chemically Competent E. coli offer:
• Highest transformation efficiencies of >5 x 10⁹ cfu/μg 
• tonA (fhuA) genotype to confer resistance to T1 and T5 phage
• Δ(ccdAB) for sensitivity to the toxic effects of the ccdB gene product, allowing negative selection of vectors containing the ccdB gene (Gateway cloning system)
• Stable F´ episome that carries lacIq repressor for inducible expression from trc, tac, and lac promoters
• Construction of more representative genomic libraries due to the elimination of mcrA, mcrBC, mrr, and hsdRMS
• lacZΔM15 for blue-white color screening of recombinant clones 
• endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I 
• recA1 for reduced occurrence of nonspecific recombination in cloned DNA

Easy-to-use One Shot format 
OmniMAX 2 T1^R Chemically Competent E. coli cells are supplied in the convenient, single-reaction One Shot format. The single-tube, single-use format allows all steps of the transformation protocol, up to plating, to take place in the same tube, thereby helping save time and prevent contamination.

Genotype
F´ [proAB+lacIqlacZΔM15 Tn10(TetR) Δ(ccdAB)] mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 supE44 thi-1 gyrA96 (NalR) relA1 tonA panD

Find the strain and format that fit your needs
• We offer other strains in chemically competent and electrocompetent cell formats to meet your specific needs.
• OmniMAX 2 T1^R and other strains are available in MultiShot formats for high throughput applications.

Order Info

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