missing translation for 'onlineSavingsMsg'
Learn More
Invitrogen™ One Shot™ TOP10F' Chemically Competent E. coli Product Code.: 10503623

Invitrogen™ One Shot™ TOP10F' Chemically Competent E. coli

Product Code. 10503623
42 x 50 μL, Each
Click to view available options
Quantity:
21 x 50 μL
42 x 50 μL
Unit Size:
Each
This item is not returnable. View return policy
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24112” to get your promotional price

Product Code. 10503623

Brand: Invitrogen™ C303006

Get 3 for 2
Use promo code 24112 at checkout
View Available Promotions

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24112” to get your promotional price

One Shot TOP10F' Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F' episome.

One Shot TOP10F´ Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F´ episome. The F´ episome carries a tetracycline resistance gene and allows isolation of single-stranded DNA (ssDNA) from vectors that have an f1 origin of replication. In addition, the F´ episome carries the lacIq repressor for inducible expression from trc, tac, and lac promoters using IPTG.

TOP10F´ competent cells are provided at a transformation efficiency of >1 x 109 cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation.

As a derivative of TOP10, TOP10F´ Chemically Competent E. coli cells carry mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr) allowing them to clone both prokaryotic and eukaryotic genomic DNA and provide efficient plasmid rescue from eukaryotic genomes. TOP10F´ cells also carry recA1 and endA1 mutations that increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

One Shot TOP10F´ Chemically Competent E. coli cells offer:
• Transformation efficiencies of >1 x 109 cfu/μg
• Stable F´ episome that allows isolation of ssDNA and carries lacIq repressor for inducible expression from trc, tac, and lac promoters
hsdR for efficient transformation of unmethylated DNA from PCR amplifications
mcrA for efficient transformation of methylated DNA from genomic preparations
lacZΔM15 for blue/white color screening of recombinant clones on plates containing X-Gal or Bluo-Gal
endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
recA1 for reduced occurrence of nonspecific recombination in cloned DNA

Note: IPTG induction is required for blue-white color screening.

Easy-to-use One Shot format
TOP10 F´ Chemically Competent E. coli cells are supplied in the convenient, single-reaction One Shot format. The single-tube, single-use format allows all steps of the transformation protocol, up to plating, to take place in the same tube, saving time and preventing contamination.

Genotype
F´{lacIq, Tn10(TetR)} mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL(StrR) endA1 nupG

Find the strains and formats that fit your needs
• DH strains are available in chemically competent and electrocompetent cell formats.
• For high throughput applications there are a variety of TOP10 strains in MultiShot formats.
• For ssDNA production try strains such as TOP10F´ (Cat. No. C303003) or electrocompetent DH12S (Cat. No. 18312017).

Order Info

Shipping Condition: Dry Ice

TRUSTED_SUSTAINABILITY

Specifications

Product Type Chemically Competent Cells
Contains F' Episome Yes
Improves Plasmid Quality Yes (endA1)
Cloning Methylated DNA Yes (mcrA)
Transformation Efficiency Level High Efficiency (>1 x 109 cfu/μg)
Content And Storage • One Shot TOP10F' Chemically Competent E. coli (42 x 50 μL)
Store Competent Cells at –80°C.

• pUC19 plasmid (50 μL at 10 pg/μL)
Store pUC19 plasmid at –20°C.

• S.O.C. Medium (6 mL)
Store S.O.C. Medium at 4°C or room temperature.

Antibiotic Resistance Bacterial Yes (Streptamycin, Tetracycline)
Cloning Unstable DNA Not suitable for cloning unstable DNA
Blue/White Screening Yes (lacZΔM15)
High-throughput Compatibility Low
Preparing Unmethylated DNA No
Reduces Recombination Yes (recA1)
Shipping Condition Dry Ice
T1 Phage - Resistant (tonA) No
Species E. coli (K12)
Promoter Trc, Tac, Lac
Format Tube
Product Line One Shot™
Quantity 42 x 50 μL
Show More Show Less

Manuals

For Research Use Only. Not for use in diagnostic procedures.

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title
Invitrogen™ One Shot™ TOP10F' Chemically Competent E. coli >

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.