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Invitrogen™ SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR.

Brand:  Invitrogen™ 10928042

5285.00 DKK valid until 2024-12-31
Use promo code "21651" to get your promotional price.

Product Code. 10606343

  • 7550.00 DKK / 100 reactions

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Description

Description

SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR. This system combines RT/Platinum Taq mix and 2X reaction mix. The RT/Platinum Taq mix contains a mixture of SuperScript II Reverse Transcriptase and Platinum Taq DNA Polymerase. The 2X reaction mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg2+ optimized for universal use, deoxyribonucleotide triphosphates, and stabilizers.

All components for cDNA synthesis and PCR are combined in a single tube with gene-specific primers and target RNA. Reverse transcription automatically follows PCR cycling without additional steps.

Note: For superior one-step RT-PCR performance, we recommend the SuperScript IV One-Step RT-PCR System (Cat. No. 12594025) or SuperScript IV One-Step RT-PCR System with ezDNase (Cat. No. 12595025). The SuperScript IV One-Step RT-PCR System combines the high processivity of SuperScript IV Reverse Transcriptase with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched product yields, specificity, and sensitivity in less time and for a broad target range, even with suboptimally pure RNA samples.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

• SuperScript II RT/Platinum Taq DNA Polymerase mix (100 μL)
• 2X Reaction mix (3 x 1 mL)
• 5 mM magnesium sulfate (500 μL)
• 50 mM magnesium sulfate (1 mL)

Store at –20°C.
Kit
Platinum Taq DNA Polymerase
1-step RT-PCR
100 rxns
Reduced
PCR Amplified cDNA
100 Reactions
Reverse Transcription
RNA
Gel Electrophoresis
1-step RT-PCR
30 to 50 min.
55°C to 60°C
SuperScript II
Wet Ice
Built-In Hot Start
Master Mix
Up to 7 kb
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For Research Use Only. Not for use in diagnostic procedures.