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Invitrogen™ pRSET A, B, & C Bacterial Expression Vectors
Designed for high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter
Brand: Invitrogen™ V35120
Includes: 20μg of each pRSET A, B and C vector is provided lyophilized. TOP10F' E. coli stab, BL21(DE3)pLysS E. coli stab, and BL21 pRSET/lacZ control E. coli stab are also included.
Description
- Expression is induced by production of T7 RNA polymerase in BL21(DE3) E. coli
- These cells also produce T7 lysozyme to reduce basal expression of target genes
- pRSET vector offers bacteriophage T7 promoter for high-level expression
- T7 gene 10 sequence to provide protein stability
- N-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-HisG Antibody
- N-terminal Xpress epitope for detection with the Anti-Xpress Antibody
- Enterokinase cleavage site for removal of fusion tag
- Each of three vectors provided (A, B, and C) has N-terminal tag coding sequence in a different reading frame relative to multiple cloning site to simplify in-frame cloning of the gene
Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
| Inducible | |
| T7 | |
| None | |
| Ampicillin (AmpR) | |
| His Tag (6x) | |
| 20 μg |
| IPTG | |
| Bacterial Expression Vector | |
| 20 μg of each pRSET A, B, & C vector is provided lyophilized. TOP10F´, BL21(DE3)pLysS, and BL21 pRSET/lacZ control E. coli stabs are also included. Store E. coli stabs at 2–8°C. All components are guaranteed stable for 6 months when properly stored. | |
| EK (Enterokinase) Recognition Site | |
| Restriction Enzyme/MCS | |
| pRSET |
For Research Use Only. Not for use in diagnostic procedures.