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Invitrogen™ Ribonuclease H

Specifically degrades RNA strand of RNA-DNA hybrid to produce 5' phosphate-terminated oligoribonucleotides and single-stranded DNA

Brand: Invitrogen™ 18021014

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Product Code. 10368002

1210.00 DKK / Each

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Includes: Ribonuclease H containing 30 units supplied at a concentration of 2U/μL

This item is not returnable. View return policy

Description

Source: Purified from E. coli expressing the E. coli RNase H gene on a plasmid
Performance and quality testing: Ribonuclease, nonspecific endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease
Unit definition: One unit hydrolyzes 1nmol of RNA in ³H-labeled poly(A)poly(dT) to acid-soluble material in 20 min. at 37°C
Unit reaction conditions: 20mM Tris-HCl (pH 7.5), 0.1M KCl, 10mM MgCl₂ , 0.1mM DTT, 5% (w/v) sucrose, 0.5nmol ³H-labeled poly(A)poly(dT), and enzyme in 50μL for 20 min. at 37°C

Removal of mRNA during second-strand cDNA synthesis; Removal of poly(A) sequences from mRNA in the presence of oligo(dT); Oligodeoxyribonucleotide-directed cleavage of RNA; PCR & Real-Time PCR; Reverse Transcription

Order Info

Shipping Conditions: Approved for shipment on wet or dry ice

Specifications

Concentration	2 U/μL
Shipping Condition	Approved for shipment on Wet or Dry Ice
Quantity	30 U

Content And Storage	1 vial of Ribonuclease H containing 30 units supplied at a concentration of 2U/μl Store at -20°C in a non-frost-free freezer.
Enzyme	RNase
Product Type	Ribonuclease H

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Product Information

Manuals

Product Sheet: Ribonuclease H Version: A.0 File size: 197.3 kB

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number3041350	Certificate TypeCertificate of Analysis	Date31/01/2025	Catalog Number18021014
Lot Number2553831	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number18021014
Lot Number2822758	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog Number18021014
Lot Number2878503	Certificate TypeCertificate of Analysis	Date26/01/2025	Catalog Number18021014
Lot Number2978833	Certificate TypeCertificate of Analysis	Date26/01/2025	Catalog Number18021014

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