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Invitrogen™ RNaseOUT™ Recombinant Ribonuclease Inhibitor

Protects mRNA and improves total cDNA yields

Brand:  Invitrogen™ 10777019

1468.32 DKK valid until 2025-03-28
Use promo code "24107" to get your promotional price.

Product Code. 10154652

  • 1956.00 DKK / Each

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Description

Description

RNaseOUT™ Recombinant Ribonuclease Inhibitor is a potent noncompetitive inhibitor of pancreatic-type ribonucleases such as RNase A, and is used to avoid RNA degradation in a variety of applications. RNaseOUT™ Recombinant Ribonuclease Inhibitor is an acidic protein with a molecular weight of ∼52 kDa. RNaseOUT™ inhibits RNase A, RNase B, and RNase C.

Applications

cDNA synthesis, RT-PCR, and in vitro transcription and translation

Source

Purified by affinity chromatography from E. coli expressing a cloned porcine gene

Performance and quality testing

SDS-PAGE purity, endodeoxyribonuclease assay, protein concentration, specific activity, performance evaluated by RT-PCR

Unit definition

One unit inhibits 5 ng of RNase A by 50% using cytidine 2', 3' cyclic monophosphate (cCMP) as the substrate

Unit reaction conditions

100 mM Tris-acetate (pH 6.5), 1 mM EDTA, 0.2 mM cCMP, 2 μg RNase A in 1 mL from 0 to 10 min at 25°C

Order Info

Shipping Condition: Dry ice

TRUSTED_SUSTAINABILITY
Specifications

Specifications

40 U/μL
10 min.
5000 units
Store at -20°C. Avoid exposing product to frequent changes in temperature. RNaseOUT™ Ribonuclease Inhibitor requires 1 mM DTT to maintain activity.

The product is guaranteed for 6 months from date of purchase unless otherwise stated in the product documentation.
RNaseOUT™
Dry Ice
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Lot Number Certificate Type Date Catalog Number
Lot Number3037375 Certificate TypeCertificate of Analysis Date31/01/2025 Catalog Number10777019,10777019_LITL18 PROMO
Lot Number2505363 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog Number10777019,10777019_LITL18 PROMO
Lot Number2478350 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog Number10777019,10777019_LITL18 PROMO
Lot Number2489605 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog Number10777019,10777019_LITL18 PROMO
Lot Number2475863 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog Number10777019,10777019_LITL18 PROMO
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Invitrogen™ RNaseOUT™ Recombinant Ribonuclease Inhibitor > 5000 units

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For Research Use Only. Not for use in diagnostic procedures.